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Series GSE94260 Query DataSets for GSE94260
Status Public on Jun 06, 2017
Title Ethylene signaling is important for isoflavonoid mediated resistance to Rhizoctonia solani in roots of Medicago truncatula
Organism Medicago truncatula
Experiment type Expression profiling by high throughput sequencing
Summary The root-infecting necrotrophic fungal pathogen Rhizoctonia solani causes significant disease to all the world’s major food crops. As a model for pathogenesis of legumes we have examined the interaction of R. solani AG8 with Medicago truncatula. RNAseq analysis of the moderately resistant M. truncatula accession A17 and highly susceptible sickle (skl) mutant (defective in ethylene sensing) identified major transcriptional reprogramming early in A17. Responses specific to A17 included components of ethylene signalling, numerous class IX ERF transcription factor family members, reactive oxygen species metabolism and consistent up-regulation of the isoflavonoid biosynthesis pathway. Mass-spectrometry revealed accumulation of the isoflavonoid related compounds liquiritigenin, formononetin, medicarpin and biochanin A in A17. Over-expression of an isoflavone synthase (IFS) in M. truncatula roots increased isoflavonoid accumulation and resistance to R. solani. Addition of exogenous medicarpin suggested this phytoalexin may be one of several isoflavonoids required to contribute to resistance to R. solani. Together these results provide evidence for the role of ethylene-mediated accumulation of isoflavonoids during defence against root pathogens in legumes.
 
Overall design Root tissue of moderately resistant wild type and highly susceptible mutant lines of Medicago truncatula responding to infection from Rhizoctona solani at two time points. Each time point contains mock treated and R. solani inoculated samples. All samples in triplicate, sequenced with strand specific illumina paired end RNA sequencing.
 
Contributor(s) Anderson J
Citation(s) 28510484
Submission date Jan 30, 2017
Last update date May 15, 2019
Contact name Jonathan P Anderson
Organization name CSIRO
Street address 147 Underwood Avenue
City Floreat
State/province Western Australia
ZIP/Postal code 6023
Country Australia
 
Platforms (2)
GPL17491 Illumina HiSeq 2000 (Medicago truncatula)
GPL23002 Illumina HiSeq 3000 (Medicago truncatula)
Samples (24)
GSM2471885 A17 Mock treated 2 dpi rep 1
GSM2471886 A17 Mock treated 2 dpi rep 2
GSM2471887 A17 Mock treated 2 dpi rep 3
Relations
BioProject PRJNA369210
SRA SRP098557

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Supplementary file Size Download File type/resource
GSE94260_Mt-Rs_Processed_data.txt.gz 555.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record
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