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| Status |
Public on Sep 12, 2017 |
| Title |
Comparison of ox-BS-450k 5-hydroxymethylcytosine and BS-450k 5-methylcytosine profiles in the human placenta |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
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| Summary |
Bisulphite (BS) and oxidative bisulphite (oxBS) converted DNA from 4 normal human placentas were hybridized to the Illumina HumanMethylation450 Beadchip v1.2, obtaining the BS and oxBS DNA methylation profiles across approximately 450,000 CpGs. By using the oxBS treatment, the selective chemical oxidation of 5-hydroxymethylcytosine (5hmC) to 5-formylcytosine (5fC) and the deamination of the latter to uracil during the BS conversion allowed the quantification of independent 5-methylcytosine (5mC) and 5hmC methylation levels at every single CpG. In consequence, this data set characterizes the genome-wide distribution of 5hmC in the human placenta offerring a high-confidence list of intervals enriched for 5hmC in this tissue.
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| Overall design |
Bisulphite and oxidative bisulphite converted DNA from 4 human placenta samples were hybridised to the Illumina HumanMethylation450 BeadChip v1.2
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| Contributor(s) |
Hernandez JR, Monk D |
| Citation(s) |
28678681 |
| |
| Submission date |
Jan 11, 2017 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Jose Ramon Hernandez |
| Organization name |
Institut d'Investigació Biomèdica de Bellvitge (IDIBELL)
|
| Department |
Cancer Epigenetics and Biology Program (PEBC)
|
| Lab |
Imprinting and Cancer group
|
| Street address |
Av. Gran Via de l'Hospitalet 199-203
|
| City |
L'Hospitalet de Llobregat, Barcelona |
| ZIP/Postal code |
08907 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (8)
|
| GSM2453247 |
bisulphite converted DNA from human placenta 3 |
| GSM2453248 |
oxidative bisulphite converted DNA from human placenta 3 |
| GSM2453249 |
bisulphite converted DNA from human placenta 5 |
| GSM2453250 |
oxidative bisulphite converted DNA from human placenta 5 |
| GSM2453251 |
bisulphite converted DNA from human placenta 64 |
| GSM2453252 |
oxidative bisulphite converted DNA from human placenta 64 |
| GSM2453253 |
bisulphite converted DNA from human placenta 67 |
| GSM2453254 |
oxidative bisulphite converted DNA from human placenta 67 |
|
| Relations |
| BioProject |
PRJNA360955 |
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