 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 30, 2016 |
| Title |
Expression data from microRNA-520f transfected PANC-1 pancreas carcinoma cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
MicroRNA-520f regulates EMT, as it activates CDH1 (mRNA) and E-cadherin (protein) expression, and it suppresses tumor cell invasion. We have characterized miR-520f target genes through whole genome transcriptional profiling of miRNA transfected pancreas cancer cells (PANC-1).
In this dataset, we include the expression data obtained from A. non-transfected (Lipofectamine RNAiMAX reagent only), B. negative control miRNA (mirVana #4464058), and C. miR-520f (mirVana #4464066) transfected PANC-1 cells (ATCC #CRL-1469). These data are used to obtain genes that are differentially expressed in response to miR-520f activity.
|
| |
|
| Overall design |
In total 9 samples (each condition in triplicate) were analyzed. We generated the following pairwise comparisons using Partek Genomics Suite 6.6 and using ANOVA. A. non-transfected vs. C. miR520, and B. neg control vs. C. miR520. Genes that were significantly up or downregulated in the miR520-treated cells in comparison to both control groups were considered miR-520f target genes.
|
| |
|
| Contributor(s) |
van Kampen JG, van Hooij O, Jansen CF, Smit FP, van Noort PI, Schultz I, Schaapveld RQ, Schalken JA, Verhaegh GW |
| Citation(s) |
28209612 |
| |
| Submission date |
Dec 28, 2016 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Gerald W Verhaegh |
| E-mail(s) |
[email protected]
|
| Phone |
+31-24-3610510
|
| Organization name |
Radboudumc
|
| Department |
Urology
|
| Lab |
URL
|
| Street address |
Geert Grooteplein Zuid 28
|
| City |
Nijmegen |
| ZIP/Postal code |
6525GA |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL5175 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version] |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA359320 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE92988_RAW.tar |
199.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...