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| Status |
Public on Mar 22, 2017 |
| Title |
Mesenchymal Transition in Human Mesothelial Cells In Vitro and Ex Vivo [TGF-β1 and IL-1β treatment] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Peritoneal dialysis (PD) is an effective form of renal replacement therapy. A significant proportion of patients who initiate PD suffer from PD-related clinical complications, including peritoneal membrane damage, which may limit the duration of treatment. Mesothelial-to-mesenchymal transition (MMT) significantly contributes to the peritoneal dysfunction related to PD. Hence, we analyzed the genetic reprograming of the MMT-process with the aim to identify new biomarkers that may be tested in PD-patients. Microarray analysis revealed a partial overlapping of MMT induced in vitro and MMT of effluent-derived mesothelial cells (ex vivo), and that MMT, both in vitro and ex vivo, is mainly a repression process being higher the number of genes that are down-regulated than those that are induced. According to cellular morphology and the number of altered genes and pathways, the MMT ex vivo could be subdivided into two stages: early/epitheliod and advanced/non-epitheliod. We could demonstrate by RT-PCR array analysis that a number of genes differentially expressed in effluent-derived non-epitheliod cells also showed significant differential expression when comparing standard versus low-GDP PD fluids. Among the secreted proteins that are up-regulated along the MMT process thrombospondin-1 (TSP1), collagen-13 (COL13), vascular endothelial growth factor A (VEGFA), and gremlin-1 (GREM1) were selected to be measured in PD effluents. TSP1, COL13 and VEGFA, but not GREM1, showed significant differences between early and advanced stages of MMT, and their expression were associated with high peritoneal transport status. The results establish a proof of concept about the feasibility of MMT-associated secreted proteins as biomarkers in PD.
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| Overall design |
Two approach: in vitro model, with two-condition experiment, HPMC vs. HPMC treated with TGF-β1 and IL-1β; ex vivo model, with three-condition experiment, HPMC vs. Epithelioid HPMC and HPMC vs. Non-Epithelioid HPMC. Biological samples: in vitro model, two control samples and two treated cells samples; ex vivo model, 1 control sample, 8 Non-Epithelioid samples and 9 Epithelioid samples. This series contains the HPMC vs. HPMC treated with TGF-β1 and IL-1β.
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| Contributor(s) |
Ruiz-Carpio V, López-Cabrera M, Sandoval P, Botías P, García-Cantalejo J |
| Citation |
Vicente Ruiz-Carpio, Pilar Sandoval, Abelardo Aguilera, Patricia Albar-Vizcaíno, María Luisa Perez-Lozano, Guadalupe T. González-Mateo, Adrían Acuña-Ruiz, Jesús García-Cantalejo, Pedro Botías, María Auxiliadora Bajo, Rafael Selgas, José Antonio Sánchez-Tomero, Jutta Passlick-Deetjen, Dorothea Piecha, Janine Büchel, Sonja Steppan and Manuel López-Cabrera. Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients. Scientific Reports 2017;7:44941. doi: 10.1038/srep44941
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| Submission date |
Dec 15, 2016 |
| Last update date |
Mar 22, 2017 |
| Contact name |
Vicente Ruiz-Carpio |
| Organization name |
Centro de Biología Molecular "Severo Ochoa"
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| Department |
Biología Celular e Inmunologia
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| Lab |
406
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| Street address |
Nicolás Cabrera, 1
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| City |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
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| Platforms (1) |
| GPL6848 |
Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Probe Name version) |
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| Samples (4)
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| GSM2429913 |
In Vitro HPMCs Treated TGF-β1 and IL-1β Sample 1 vs In Vitro Control HPMCs Sample 1 Replicate 1 |
| GSM2429914 |
In Vitro HPMCs Treated TGF-β1 and IL-1β Sample 1 vs In Vitro Control HPMCs Sample 1 Replicate 2 |
| GSM2429915 |
In Vitro HPMCs Treated TGF-β1 and IL-1β Sample 2 vs In Vitro Control HPMCs Sample 2 Replicate 1 |
| GSM2429916 |
In Vitro HPMCs Treated TGF-β1 and IL-1β Sample 2 vs In Vitro Control HPMCs Sample 2 Replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE92455 |
Mesenchymal Transition in Human Mesothelial Cells In Vitro and Ex Vivo |
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| Relations |
| BioProject |
PRJNA357607 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE92454_RAW.tar |
49.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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