 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jan 22, 2008 |
| Title |
Gene expression in E.coli K-12 MC4100 at 37˚C and 23˚C in M9 glycerol exponential phase growth |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
|
| Summary |
Because many virulence genes in E. coli and other pathogens are regulated by temperature, we wanted to determine on a genome-wide scale which genes are modulated in adapting to both human host temperature (37˚C) and ambient room temperature (23˚C). Overall, 126 genes were found to be more highly expressed at 37˚C (1) whereas 297 genes were more highly expressed at 23˚C (2). Genes involved in the uptake and utilization of amino acids, carbohydrates, and iron dominated the 37˚C list, supporting a model in which temperature serves as a host cue to increase expression of bacterial genes needed for growth. 122 of the 297 genes more highly expressed at 23˚C are RpoS-controlled, confirming genome-wide the model that low temperature serves as a primary cue to trigger the general stress response. Several genes expressed at 23˚C overlap with the cold shock response, suggesting that strategies used to adapt to sudden shifts in temperature also mediate long-term growth at 23˚C. Another category of genes more highly expressed at 23˚C are associated with biofilm development, implicating temperature as an important cue influencing this developmental pathway.
1.) White-Ziegler, C. A., A. J. Malhowski, and S. D. Young. Human body temperature (37˚C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12. Journal of Bacteriology 2007. Vol. 189(15):5429-40. Epub 2007 May 25.
2.)White-Ziegler, C. A., S. Um, N.Peréz, A. L. Berns, A. J. Malhowski, and S. Young. Biofilm, coldshock, and RpoS-dependent genes demonstrate increased expression during low temperature growth (23˚C) in Escherichia coli K-12. In revision for Microbiology.
Keywords: stress response
|
| |
|
| Overall design |
Growth and medium conditions were chosen (M9 glycerol medium with aeration) that maximize papBA transcription at 37˚C so we could use this operon as a temperature-regulated positive control in our microarrays. Parallel cultures grown at 37˚C and 23˚C were harvested in exponential phase (OD600=0.2-0.6) at 9-11 generations growth after inoculation. Thus, the results presented here reflect the adapted state and signify genes whose expression is differentially maintained over long-term growth at a given temperature.
1.) White-Ziegler, C. A., A. J. Malhowski, and S. D. Young. Human body temperature (37˚C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12. Journal of Bacteriology 2007. Vol. 189(15):5429-40. Epub 2007 May 25.
|
| |
|
| Contributor(s) |
Whi CA, Malhowski AJ, Young SD |
| Citation(s) |
17526711, 18174134 |
| |
| Submission date |
Sep 30, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Christine Ann White-Ziegler |
| E-mail(s) |
[email protected]
|
| Phone |
413-585-3815
|
| Organization name |
Smith College
|
| Department |
Biological Sciences
|
| Lab |
White-Ziegler
|
| Street address |
Clark Science Center
|
| City |
Northampton |
| State/province |
MA |
| ZIP/Postal code |
01063 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5942 |
Univerisity of Wisconsin Gene Expression Center_Escherichia coli_4.2K_Eco15 |
|
| Samples (5)
|
| GSM232846 |
E.coli K-12_MC4100_37˚C and 23˚C M9 glycerol_Rep1 |
| GSM232847 |
E.coli K-12_MC4100_37˚C and 23˚C M9 glycerol_Rep2 |
| GSM232848 |
E.coli K-12_MC4100_37˚C and 23˚C M9 glycerol_Rep3 |
| GSM232849 |
E.coli K-12_MC4100_37˚C and 23˚C M9 glycerol_Rep4 |
| GSM232850 |
E.coli K-12_MC4100_37˚C and 23˚C M9 glycerol_Rep5 |
|
| Relations |
| BioProject |
PRJNA102773 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9197_RAW.tar |
3.7 Mb |
(http)(custom) |
TAR (of GPR) |
| GSE9197_normalized_log_ratios_for_uncondensed_platform.txt |
381.9 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...