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Series GSE919

Query DataSets for GSE919

Status

Public on Dec 30, 2003

Title

Comparison of WT and YODA

Organism

Arabidopsis thaliana

Experiment type

Expression profiling by array

Summary

Wild type and yda-2 plants were germinated and grown on commercial potting mix in walk-in chambers with constant illumination (~150 micromoles per square meter per second) at 22oC. The aerial parts of the plants were harvested at the rosette stage, prior to bolting. Total RNA was prepared using Trizol reagent (Invitrogen) with the additional steps recommended for polysaccharide-rich tissues. Hybridization probes for the GeneChip AtGenome1 microarrays (Affymetrix) were prepared from 20 mg total RNA. All procedures followed Affymetrix protocols. Briefly, cDNA was synthesized using Superscript reverse transcriptase (Invitrogen) and a T7-(dT)24 primer, purified by phenol/chloroform extraction using Phase Lock Gel tubes (Eppendorf), and precipitated with ethanol. Labeled antisense RNA was generated with a BioArray kit (Enzo) and purified using an RNeasy kit (Qiagen). 25 mg of the fragmented cRNAs were used for hybridization to the arrays. Affymetrix instruments were used for hybridization, washing, staining and scanning of the arrays according to the provided instructions.
Data from four biological replications representing tissue grown and harvested at different times was collected. The average signals of all eight experiments were scaled to the target value of 500.

Keywords = yoda
Keywords = yda
Keywords = kinase
Keywords = MAPKKK
Keywords: parallel sample

Contributor(s)

Lukowitz W, Roeder A, Parmenter D, Somerville C

Citation(s)

14718171

Submission date

Dec 19, 2003

Last update date

Mar 02, 2012

Contact name

Chris Somerville

E-mail(s)

[email protected]