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| Status |
Public on Mar 27, 2017 |
| Title |
Cbx3 Maintains Lineage Specificity During Neural Differentiation [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Cbx3 (HP1γ) that is a member of the heterochromatin protein 1 familiy enriched in gene bodies of pluripotent ESCs but promoters of differentiated pre-iPSCs. To determine whether Cbx3 becomes enriched at promoters to influence gene regulation during differentiation, we converted ESCs to NPCs in monolayer culuture and performed genomewide ChIP-seq of Cbx3. Our results shows that Cbx3 enriches at the promoters of genes upon differentiation of ESCs to NPCs and stablizes the binding of Mediator subunit Med26 to pre-initiation complex (PIC) in NPCs.
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| Overall design |
Examine the enrichment of Cbx3 and Pol II in wild-type ESC-derived NPCs, and the enrichment of Med26, Cdk8 and Pol II in NPC transfected with control siRNA targeting luciferase or siRNA mediated knockdown of Cbx3 by ChIP-seq analysis.
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| Contributor(s) |
Huang C, Su T, Carey M, Kurdistani S |
| Citation(s) |
28270516 |
| |
| Submission date |
Nov 05, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Siavash K Kurdistani |
| E-mail(s) |
[email protected]
|
| Organization name |
UCLA
|
| Department |
Biological Chemistry
|
| Lab |
Kurdistani
|
| Street address |
615 Charles E Young Dr South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE89575 |
Cbx3 Maintains Lineage Specificity During Neural Differentiation |
|
| Relations |
| BioProject |
PRJNA352607 |
| SRA |
SRP092645 |
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