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| Status |
Public on Jul 22, 2009 |
| Title |
Global expression profiling to study the effect of triazolopyrimidine herbicide treatment on Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
Using whole genome microarray (Affymetrix ATH1) we studied the transcriptional response of Arabidopsis thaliana to triazolopyrimidine (FirstRate) herbicde that inhibits acetolactate synthase (ALS) enzyme and thus disrupts branched chain amino acid biosynthesis. A number of genes related to amino acid, protein metabolism, growth, regulatory networks, respiratory pathways, stress, defense and secondary metabolism were altered.
Keywords: Acetolactate synthase (ALS) inhibiting herbicide stress response
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| Overall design |
Surfactant (preference 0.25%) treated plants were used as carrier control group and EC50 concentration of triazolopyrimidine was used as the herbicide treatment group. Each of the control and treatment group consisted of 3 biological replicates and each biological replicates comprised leaves from 10 individual plants. RNA was extracted at 24h post treatment to study the transcriptional alterations caused by the herbicide treatment.
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| Contributor(s) |
Das M, Mader MT, Reichman JR, Watrud LS, Pfleeger TG, Schäffner AR, Olszyk DM |
| Citation(s) |
20043233 |
| |
| Submission date |
Aug 31, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Malay Das |
| E-mail(s) |
[email protected]
|
| Phone |
5417544315
|
| Fax |
5417544799
|
| Organization name |
National Research Council, US-EPA
|
| Department |
EEB
|
| Street address |
200 SW 35th STreet
|
| City |
Corvallis |
| State/province |
OR |
| ZIP/Postal code |
97333 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
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| GSM226322 |
Carrier biological rep1 (Triazolopyrimidine) |
| GSM226323 |
Carrier biological rep2 (Triazolopyrimidine) |
| GSM226324 |
Carrier biological rep3 (Triazolopyrimidine) |
| GSM226325 |
Triazolopyrimidine EC50 biological rep1 |
| GSM226326 |
Triazolopyrimidine EC50 biological rep2 |
| GSM226327 |
Triazolopyrimidine EC50 biological rep3 |
|
| Relations |
| BioProject |
PRJNA102337 |
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