|
| Status |
Public on Dec 21, 2016 |
| Title |
Identification of a U/Zn/Cu responsive global regulatory two-component system in Caulobacter crescentus [RNA-seq] |
| Organism |
Caulobacter vibrioides |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Despite the well-known toxicity of uranium (U) to bacteria, little is known about how cells sense and respond to U. The recent finding of a U-specific stress response in Caulobacter crescentus has provided a foundation for studying the mechanisms of U- perception in bacteria. To gain insight into this process, we used a forward genetic screen to identify the regulatory components governing expression of the urcA promoter (PurcA) that is strongly induced by U. This approach unearthed a previously uncharacterized two-component system, UzcRS, which is responsible for U-dependent activation of PurcA. UzcRS is also highly responsive to zinc and copper, revealing a broader specificity than previously thought. Using ChIP-seq, we found that UzcR binds extensively throughout the genome in a metal-dependent manner and recognizes a non-canonical DNA binding site. Coupling the genome-wide occupancy data with RNA-seq analysis revealed that UzcR is a global regulator of transcription, predominately activating genes encoding proteins that are localized to the cell envelope; these include metallopeptidases, multidrug resistant efflux (MDR) pumps, TonB-dependent receptors and many proteins of unknown function. Collectively, our data suggest that UzcRS couples detection of U, Zn and Cu with a novel extracytoplasmic stress response.
|
| |
|
| Overall design |
WT and uzcR mutant strains assayed in the presence and absence of Zn in triplicate
|
| |
|
| Contributor(s) |
Park D, Jiao Y |
| Citation(s) |
28035693 |
| |
| Submission date |
Sep 21, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Dan Park |
| E-mail(s) |
[email protected]
|
| Phone |
7207710906
|
| Organization name |
Lawrence Livermore National Lab
|
| Department |
BBTD
|
| Street address |
7000 East Ave
|
| City |
Livermore |
| State/province |
CA |
| ZIP/Postal code |
94550 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL22063 |
Illumina HiSeq 4000 (Caulobacter vibrioides) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE87173 |
Identification of a U/Zn/Cu responsive global regulatory two-component system in Caulobacter crescentus |
|
| Relations |
| BioProject |
PRJNA343782 |
| SRA |
SRP090233 |
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