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| Status |
Public on Nov 01, 2017 |
| Title |
mRNA expression profiling after Ago2-immunoprecipitation (IP) in unstimulated and TGF-β1-stimulated primary parenchymal lung fibroblasts of two control subjects |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
To identify the target gene repertoire of miRNAs (i.e. the miRNA-targetome) of unstimulated and TGF-β1-stimulated primary parenchymal lung fibroblasts, Ago2-IP was performed followed by mRNA expression profiling.
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| Overall design |
The Ago2-IP was performed on the unstimulated and TGF-β1-stimulated primary parenchymal lung fibroblasts of two control subjects. The RNA of the Total (T) fraction and the immunoprecipitated (IP) fraction were isolated using miRNeasy Mini Kit and miRNeasy Micro Kit, respectively. Subsequently, the RNA was labeled with Cyanine 3 (Cy3) and Cyanine 5 (Cy5) in a dye-swap experiment and cRNA was generated which was purified using the RNeasy Mini Kit. The labeled and amplified cRNA was then used to perform the mRNA expression profiling using the G3 Human Gene Expression 8x60K v3 Microarray.
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| Contributor(s) |
Ong J, Timens W, Rajendran V, Algra A, Spira A, Lenburg ME, Campbell JD, van den Berge M, Postma DS, van den Berg A, Kluiver J, Brandsma C |
| Citation(s) |
28910321 |
| |
| Submission date |
Aug 29, 2016 |
| Last update date |
Feb 20, 2018 |
| Contact name |
Jennie Ong |
| E-mail(s) |
[email protected]
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| Organization name |
University Medical Center Groningen
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| Street address |
Hanzeplein 1
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| City |
Groningen |
| ZIP/Postal code |
9713 GZ |
| Country |
Netherlands |
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| Platforms (1) |
| GPL21185 |
Agilent-072363 SurePrint G3 Human GE v3 8x60K Microarray 039494 [Probe Name Version] |
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| Samples (16)
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| GSM2297053 |
IP1 of primary parenchymal lung fibroblasts of control 1 |
| GSM2297054 |
T1 fraction of primary parenchymal lung fibroblasts of control 1 |
| GSM2297055 |
T2 fraction of primary parenchymal lung fibroblasts of control 1 |
| GSM2297056 |
IP2 of primary parenchymal lung fibroblasts of control 1 |
| GSM2297057 |
IP1 of primary parenchymal lung fibroblasts of control 2 |
| GSM2297058 |
T1 fraction of primary parenchymal lung fibroblasts of control 2 |
| GSM2297059 |
T2 fraction of primary parenchymal lung fibroblasts of control 2 |
| GSM2297060 |
IP2 of primary parenchymal lung fibroblasts of control 2 |
| GSM2536196 |
IP1 of primary parenchymal lung fibroblasts of control 1 stimulated with TGF-β1 |
| GSM2536197 |
T1 fraction of primary parenchymal lung fibroblasts of control 1 stimulated with TGF-β1 |
| GSM2536198 |
T2 fraction of primary parenchymal lung fibroblasts of control 1 stimulated with TGF-β1 |
| GSM2536199 |
IP2 of primary parenchymal lung fibroblasts of control 1 stimulated with TGF-β1 |
| GSM2536200 |
IP1 of primary parenchymal lung fibroblasts of control 2 stimulated with TGF-β1 |
| GSM2536201 |
T1 fraction of primary parenchymal lung fibroblasts of control 2 stimulated with TGF-β1 |
| GSM2536202 |
T2 fraction of primary parenchymal lung fibroblasts of control 2 stimulated with TGF-β1 |
| GSM2536203 |
IP2 of primary parenchymal lung fibroblasts of control 2 stimulated with TGF-β1 |
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| This SubSeries is part of SuperSeries: |
| GSE86186 |
miRNA expression data before and after TGF-β treatment and Ago2-associated transcripts in lung fibroblasts |
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| Relations |
| BioProject |
PRJNA340782 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE86183_US45102948_257236310965_S01_GE2_1200_Jun14_1_1.txt.gz |
6.0 Mb |
(ftp)(http) |
TXT |
| GSE86183_US45102948_257236310965_S01_GE2_1200_Jun14_1_2.txt.gz |
6.0 Mb |
(ftp)(http) |
TXT |
| GSE86183_US45102948_257236310965_S01_GE2_1200_Jun14_1_3.txt.gz |
6.0 Mb |
(ftp)(http) |
TXT |
| GSE86183_US45102948_257236310965_S01_GE2_1200_Jun14_1_4.txt.gz |
6.0 Mb |
(ftp)(http) |
TXT |
| GSE86183_US45102948_257236310965_S01_GE2_1200_Jun14_2_1.txt.gz |
6.0 Mb |
(ftp)(http) |
TXT |
| GSE86183_US45102948_257236310965_S01_GE2_1200_Jun14_2_2.txt.gz |
5.9 Mb |
(ftp)(http) |
TXT |
| GSE86183_US45102948_257236310965_S01_GE2_1200_Jun14_2_3.txt.gz |
6.0 Mb |
(ftp)(http) |
TXT |
| GSE86183_US45102948_257236310965_S01_GE2_1200_Jun14_2_4.txt.gz |
6.0 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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