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| Status |
Public on Oct 29, 2007 |
| Title |
NFkB in multiple myeloma - EJM IkBa super-repressor |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Mechanisms of constitutive NF-kappaB signaling in multiple myeloma are unknown. An inhibitor of IkappaB kinase beta (IKKbeta), targeting the classical NF-kappaB pathway, was lethal to many myeloma cell lines. Several had elevated expression of NIK due to genomic alterations or enhanced protein stability while others had inactivating mutations or deletion of TRAF3. Both abnormalities triggered the classical and alternative NF-kappaB pathways. A majority of primary myeloma patient samples and cell lines had elevated NF-kappaB target gene expression, often associated with genetic and epigenetic alteration of NIK, TRAF3, CYLD, BIRC2/BIRC3, CD40, NFKB1, and NFKB2. These genetic and functional data demonstrate that addiction to the NF-kappaB pathway is a frequent feature of myeloma and suggest that IKKbeta inhibitors hold promise for the treatment of this disease.
Keywords: time series design
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|
| Overall design |
EJM cells were treated with doxycycline to induce the IkappaBalpha super-repressor for 1-4 days. A total of 4 samples were analyzed in comparison to uninduced cells.
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| Citation(s) |
17692804 |
| |
| Submission date |
Jul 13, 2007 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Christina Annunziata |
| E-mail(s) |
[email protected]
|
| Phone |
301-402-7259
|
| Fax |
301-496-9956
|
| Organization name |
Center for Cancer Research
|
| Department |
Metabolism Branch
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-1374 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (4)
|
| GSM210212 |
IkB-SR induced EJM - 1 day - mAdbID:79133 |
| GSM210213 |
IkB-SR induced EJM - 2 days - mAdbID:79134 |
| GSM210214 |
IkB-SR induced EJM - 3 days - mAdbID:79135 |
| GSM210215 |
IkB-SR induced EJM - 4 days - mAdbID:79136 |
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| This SubSeries is part of SuperSeries: |
| GSE8487 |
Mechanisms of constitutive NF-kappaB signaling in multiple myeloma |
|
| Relations |
| BioProject |
PRJNA105363 |
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