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| Status |
Public on Sep 01, 2007 |
| Title |
Non-additive and imprinted gene expression in hybrid maize endosperm_13DAP |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by array
|
| Summary |
The nuclear content of the plant endosperm is the result of the contribution two maternal genomes and a single paternal genome. This 2:1 dosage relationship provides a unique system for studying the additivity of gene expression levels in reciprocal hybrids. A combination of microarray profiling and allele-specific expression analysis was performed using RNA isolated from endosperm tissues of maize inbred lines B73 and Mo17 and their reciprocal hybrids at two developmental stages, 13 and 19 days after pollination. By assessing the relative levels of expression in the reciprocal hybrids it was possible to determine the prevalence of additive and non-additive expression patterns. While the majority of differentially expressed genes displayed additive expression patterns in the endosperm, approximately 10% of the genes displayed non-additive expression patterns including maternal-like, paternal-like, dominant high-parent, dominant low-parent and expression patterns outside the range of the inbreds. The frequency of hybrid expression patterns outside of the parental range in maize endosperm tissue is much higher than that observed for vegetative tissues. For a set of 90 genes allele-specific expression assays were employed to monitor allelic bias and regulatory variation. Eight of these genes exhibited evidence for maternally or paternally biased expression at multiple stages of endosperm development and are potential examples of differential imprinting. Collectively, our data indicate that parental effects on gene expression are much stronger in endosperm than in vegetative tissues, and that endosperm imprinting may be far more common than previously estimated.
Keywords: genotype comparison
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| Overall design |
Affymetrix microarrays were used to perform expression profiling on 13 day after pollination endosperm tissue of four different genotypes; B73; Mo17, B73xMo17 and Mo17xB73. There are three biological replicates for each of the tissues. Each biological sample represents a pool containing 5 endosperms each, from 6 different ears.
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| Contributor(s) |
Springer NM, Stupar RM, Hermanson PJ |
| Citation(s) |
17766400 |
| |
| Submission date |
Jun 25, 2007 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Nathan M Springer |
| E-mail(s) |
[email protected]
|
| Phone |
6126246241
|
| Fax |
6126251738
|
| Organization name |
University of Minnesota
|
| Department |
Plant Biology
|
| Street address |
1445 Gortner Ave
|
| City |
Saint Paul |
| State/province |
MN |
| ZIP/Postal code |
55108 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL4032 |
[Maize] Affymetrix Maize Genome Array |
|
| Samples (12)
|
| GSM205365 |
Mo17 13 DAP endosperm tissue biological replicate 1 |
| GSM205366 |
B73 13 DAP endosperm tissue biological replicate 1 |
| GSM205367 |
Mo17xB73 13 DAP endosperm tissue biological replicate 1 |
| GSM205368 |
B73xMo17 13 DAP endosperm tissue biological replicate 1 |
| GSM205369 |
Mo17 13 DAP endosperm tissue biological replicate 2 |
| GSM205370 |
B73 13 DAP endosperm tissue biological replicate 2 |
| GSM205371 |
Mo17xB73 13 DAP endosperm tissue biological replicate 2 |
| GSM205372 |
B73xMo17 13 DAP endosperm tissue biological replicate 2 |
| GSM205373 |
Mo17 13 DAP endosperm tissue biological replicate 3 |
| GSM205374 |
B73 13 DAP endosperm tissue biological replicate 3 |
| GSM205375 |
Mo17xB73 13 DAP endosperm tissue biological replicate 3 |
| GSM205376 |
B73xMo17 13 DAP endosperm tissue biological replicate 3 |
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| This SubSeries is part of SuperSeries: |
| GSE8308 |
Non-additive and imprinted gene expression in hybrid maize endosperm_13DAP and 19DAP |
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| Relations |
| BioProject |
PRJNA105427 |
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