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| Status |
Public on Aug 17, 2007 |
| Title |
Identification of hypoxia-inducible genes in Arabidopsis mesophyll cells |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The goal of this experiment was to investigate the early transcript changes (6h) induced by hypoxia treatment in mesophyll protoplasts. A single pair (control & hypoxia) of GeneChips® was used to confirm that hypoxia treatment altered the expression of an overlapping set of genes controlled by KIN10 (At3g01090) in Arabidopsis mesophyll protoplasts.
Keywords: KIN10, KIN11, darkness, hypoxia, starvation, stress, sugar signalling, Arabidopsis, SnRK1
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| Overall design |
For the hypoxic treatment protoplasts were submerged in mannitol buffer and the hypoxia condition was confirmed by the activation of the well-established early marker genes ADH (alcohol dehydrogenase, At1g77120) and PDC1 (pyruvate decarboxylase-1, At4g33070) based on RT-PCR and GeneChip® analyses. Results show a clear overlap in genes induced under hypoxic conditions and those induced by KIN10 expression in protoplasts. The overlap of the gene expression profiles was confirmed by triplicated real-time quantitative RT-PCR analyses of selected marker genes.
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| Contributor(s) |
Baena-Gonzalez E, Rolland F, Thevelein JM, Sheen J |
| Citation(s) |
17671505 |
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| Submission date |
Jun 22, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Elena Baena-Gonzalez |
| E-mail(s) |
[email protected]
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| Organization name |
Massachusetts General Hospital and Harvard Medical School
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| Department |
Molecular Biology (MGH) and Department of Genetics (HMS)
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| Lab |
Jen Sheen
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| Street address |
Simches Research Building, 185 Cambridge Street, CPZN7250
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114-2790 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA101191 |
