The purpose is to obtain samples for microRNA analysis in human U937 cells infected with Zaire Ebola virus wild-type in the ΔVP30 background and Δmucin virus.
Overall design
Human U937 cells (monocyte-like) expressing the Ebola VP30 protein were infected with Zaire Ebola virus wild-type (wild) in the ΔVP30 background and Δmucin virus (encodes a GP lacking the mucin domain) (mucin) in the Ebola ΔVP30 background. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 8, 18, and 30 h post-infection.
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