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| Status |
Public on Aug 10, 2017 |
| Title |
Fractionation iCLIP detects persistent SR protein binding to conserved, retained introns in chromatin, nucleoplasm and cytoplasm |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
We report a new method which combines subcellular fractionation to advance sequencing technique iCLIP. In this way we identified the spectrum of interactions of two SR-proteins (SRSF3 and SRSF7) to their target RNAs in different subcellular compartments
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| Overall design |
Examination of 4 replicates for Cytoplasm, Nucleoplasm and Chromatin fractions using Fr-iCLIP technique for SRSF3-GFP; examination of 3 replicates for Cytoplasm, Nucleoplasm and Chromatin fractions using Fr-iCLIP for SRSF7-GFP
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| Contributor(s) |
Brugiolo M, Botti V, Liu N, Müller-McNicoll M, Neugebauer KM |
| Citation(s) |
28977534 |
| |
| Submission date |
Mar 31, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Mattia Brugiolo |
| Organization name |
Yale University
|
| Department |
Molecular biophysics and biochemistry
|
| Lab |
Karla M. Neugebauer's lab
|
| Street address |
333 Cedar Street
|
| City |
New Haven |
| State/province |
Connecticut |
| ZIP/Postal code |
06520 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA317002 |
| SRA |
SRP072700 |
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