|
| Status |
Public on May 31, 2016 |
| Title |
Comparative transcriptomics of Clostridium thermocellum wild type (Δhpt) and Type I glutamine synthetase (Clo1313_2031) deletion strains using RNA-seq |
| Organism |
Acetivibrio thermocellus DSM 1313 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-seq analysis revealed that genes involved in urea uptake and metabolism were significantly upregulated in the Clo1313_2031 deletion strain, suggesting that deletion of Clo1313_2031 mimics nitrogen starvation in C. thermocellum. Additionally, genes encoding the RNF-complex were also more highly expressed and in turn may have a potential role in increasing ethanol production.
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| |
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| Overall design |
Samples for RNA-seq were taken from mid-exponential phase (OD ~ 0.33) batch cultures grown in MTC on 4.5 g/l cellobiose
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| |
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| Contributor(s) |
Rydzak T, Guss A, Klingeman DM, Brown SD, Garcia D |
| Citation(s) |
28400329 |
| |
| Submission date |
Feb 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Dawn Marie Klingeman |
| E-mail(s) |
[email protected]
|
| Phone |
+18655763435
|
| Organization name |
Oak Ridge National Lab
|
| Department |
Biosciences Division
|
| Lab |
RNA Profiling
|
| Street address |
1 Bethel Valley Rd building 15056 Rm 366 MS 6038
|
| City |
Oak Ridge |
| State/province |
TN |
| ZIP/Postal code |
37831-6342 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20122 |
Illumina HiSeq 2500 (Ruminiclostridium thermocellum DSM 1313) |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA312946 |
| SRA |
SRP070709 |
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