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| Status |
Public on Nov 22, 2018 |
| Title |
Gene expression analysis in grape berries at veraison (SIB_06) |
| Organism |
Vitis vinifera |
| Experiment type |
Expression profiling by array
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| Summary |
A bunch of grapes at veraison is comprised of berries at different stages of development ranging from hard green to soft red. Veraison represents an important transition stage in the ripening process where key genes may be switched on or off.
Single berries at various stages of development from hard green to soft red were collected at a single time point from a single bunch of grapes going through veraison. Gene expression patterns were obtained from single berries representing different developmental stages.
Keywords: Developmental series
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| Overall design |
Six different developmental stages were analysed for gene expression changes using the Affymetrix one colour array platform. The developmental stages were defined based on berry softness and colour. They were hard green, soft green, blush, soft red. Hard green berries were divided further into two categories based on their softness. Four biological replicates were collected for the firmer hard green berries and two biological replicates were collected for the less firm hard green berries. Two biological replicates were collected for each of the other developmental stages. Biotin labeled probes were prepared from the extracted RNA from each deseeded berry and hybridised to the Vitis vinifera GeneChip. The sample/GeneChip position on the Affymetrix hybridisation station was randomised using the AlgDesign package from R.
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| Contributor(s) |
Thomas MR, Davies C, Iocco P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 07, 2007 |
| Last update date |
Nov 22, 2018 |
| Contact name |
Mark Raymond Thomas |
| E-mail(s) |
[email protected]
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| Organization name |
CSIRO
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| Department |
Plant Industry
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| Street address |
PO Box 350
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| City |
Glen Osmond |
| State/province |
SA |
| ZIP/Postal code |
5064 |
| Country |
Australia |
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| Platforms (1) |
| GPL1320 |
[Vitis_Vinifera] Affymetrix Vitis vinifera (Grape) Genome Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA99873 |
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