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| Status |
Public on Nov 02, 2016 |
| Title |
Differential gene expression in neuroblastoma cells after transfection with control siRNA, MYCN siRNA or TFAP4 siRNA. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We analyed the gene expression profiles after knocking down MYCN or TFAP4. Results showed that transcription factor MYCN and TFAP4 commonly regulats a subset of genes that may contribute to neuroblastoma cells proliferation and migration.
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| |
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| Overall design |
Neuroblastoma BE(2)-C cells were transfected with control siRNA, MYCN siRNA1, MYCN siRNA2, TFAP4 siRNA1 and TFAP4 siRNA2, respectively. Total RNA was extracted from cells 30 hours after each transfection and subjected to gene expression study by Affymetrix microarray.
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| |
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| Contributor(s) |
Xue C, Liu T |
| Citation(s) |
27448979 |
| |
| Submission date |
Nov 03, 2015 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Chengyuan Xue |
| E-mail(s) |
[email protected]
|
| Phone |
61-2-93851562
|
| Organization name |
Children's Cancer Institute
|
| Department |
Molecular Diagnostics
|
| Lab |
MD
|
| Street address |
PO Box 81
|
| City |
Randwick |
| State/province |
NSW |
| ZIP/Postal code |
2031 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA301034 |
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