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| Status |
Public on Jan 16, 2017 |
| Title |
Gene expression profiling of cell subpopulations from different regions of the mouse brain |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
As a starting point for dissecting the cellular heterogeneity of astrocytes in the adult brain, we focused our initial studies on the olfactory bulb (OB) and combined an Aldh1l1-GFP reporter mouse with candidate cell surface markers to isolate astrocyte sub-populations using FACS analysis. To this end, we screened a panel of cell surface antigens for their ability to divide the Aldh1l1-GFP astrocyte population within the OB into distinct sub-populations. Candidate cell surface markers were selected based on previously published gene expression profiling data from our lab and others and antibody availability. Different subpopulations from different brain regions were profiled for gene expression.
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| Overall design |
Gene expression profiles (by RNA-seq) were taken of mouse cell lines in 6 populations and 3 brain regions.
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| Contributor(s) |
Creighton C, Chen F, Deneen B |
| Citation(s) |
28166219 |
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| Submission date |
Sep 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Chad Creighton |
| E-mail(s) |
[email protected]
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| Organization name |
Baylor College of Medicine
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| Department |
Biostatistics, Ducan Cancer Center
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| Street address |
One Baylor Plaza, Mail Stop: BCM305
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (36)
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| GSM1873197 |
sample bi43314, brain region #1, population:Neg |
| GSM1873198 |
sample bi43315, brain region #1, population:Neg |
| GSM1873199 |
sample bi43317, brain region #2, population:Neg |
| GSM1873200 |
sample bi43321, brain region #2, population:Neg |
| GSM1873201 |
sample bi43322, brain region #3, population:Neg |
| GSM1873202 |
sample bi43323, brain region #3, population:Neg |
| GSM1873203 |
sample bi43327, brain region #1, population:A |
| GSM1873204 |
sample bi43328, brain region #1, population:A |
| GSM1873205 |
sample bi43333, brain region #2, population:A |
| GSM1873206 |
sample bi43637, brain region #2, population:A |
| GSM1873207 |
sample bi43337, brain region #3, population:A |
| GSM1873208 |
sample bi43638, brain region #3, population:A |
| GSM1873209 |
sample bi43343, brain region #1, population:B |
| GSM1873210 |
sample bi43344, brain region #1, population:B |
| GSM1873211 |
sample bi43348, brain region #2, population:B |
| GSM1873212 |
sample bi43349, brain region #2, population:B |
| GSM1873213 |
sample bi43352, brain region #3, population:B |
| GSM1873214 |
sample bi43639, brain region #3, population:B |
| GSM1873215 |
sample bi43357, brain region #1, population:C |
| GSM1873216 |
sample bi43358, brain region #1, population:C |
| GSM1873217 |
sample bi43363, brain region #2, population:C |
| GSM1873218 |
sample bi43364, brain region #2, population:C |
| GSM1873219 |
sample bi43368, brain region #3, population:C |
| GSM1873220 |
sample bi43369, brain region #3, population:C |
| GSM1873221 |
sample bi43640, brain region #1, population:D |
| GSM1873222 |
sample bi43641, brain region #1, population:D |
| GSM1873223 |
sample bi43642, brain region #2, population:D |
| GSM1873224 |
sample bi43643, brain region #2, population:D |
| GSM1873225 |
sample bi43644, brain region #3, population:D |
| GSM1873226 |
sample bi43645, brain region #3, population:D |
| GSM1873227 |
sample bi43646, brain region #1, population:E |
| GSM1873228 |
sample bi43647, brain region #1, population:E |
| GSM1873229 |
sample bi43648, brain region #2, population:E |
| GSM1873230 |
sample bi43649, brain region #2, population:E |
| GSM1873231 |
sample bi43650, brain region #3, population:E |
| GSM1873232 |
sample bi43651, brain region #3, population:E |
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| Relations |
| BioProject |
PRJNA295171 |
| SRA |
SRP063505 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE72826_RAW.tar |
49.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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