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| Status |
Public on Sep 16, 2015 |
| Title |
UG26-1B6 stroma-derived factors regulate hematopoietic stem cell maintenance |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Hematopoietic stem cell (HSC) are regulated by their ’niche’, which limits activation of HSCs, to ensure their maintenance and self-renewal.
To dissect the interactions involved during rapid activation in response to stress, we used cell co-culture as a model. Here, we studied gene expression changes of UG26-1B6 stromal cells in response to the presence of Lineage- Sca-1+ Kit+ (LSKs).
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| Overall design |
UG26-1B6 stromal cells were used in a total of 5 conditions (before co-culture (d0), after one (d1; 24 h), two (d2) and three (d3) days of co-culture with Lineage- Sca-1+ Kit+ (LSKs), after one (d1; 24 h) day of culture medium change (control)) with 2-3 technical replicates per condition.
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| Contributor(s) |
Vilne B, Prazeres da Costa O, Peschel C, Oostendorp RA |
| Citation(s) |
26527384 |
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| Submission date |
May 13, 2015 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Robert A.J. Oostendorp |
| Organization name |
III. Medizinische Klinik und Poliklinik
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| Department |
Klinikum Rechts der Isar Technische Universität München
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| Lab |
Laboratory of Stem Cell Physiology
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| Street address |
Trogerstrasse 32
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| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
81675 |
| Country |
Germany |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (13)
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| GSM1682813 |
UG26-1B6 stromal cells before co-culture (d0), biol rep1 |
| GSM1682814 |
UG26-1B6 stromal cells before co-culture (d0), biol rep2 |
| GSM1682815 |
UG26-1B6 stromal cells before co-culture (d0), biol rep3 |
| GSM1682816 |
UG26-1B6 stromal cells after one (d1; 24h) day of culture medium change (control), biol rep1 |
| GSM1682817 |
UG26-1B6 stromal cells after one (d1; 24h) day of culture medium change (control), biol rep2 |
| GSM1682818 |
UG26-1B6 stromal cells after one (d1; 24h) day of culture co-culture with Lineage- Sca-1+ Kit+ (LSKs), biol rep1 |
| GSM1682819 |
UG26-1B6 stromal cells after one (d1; 24h) day of culture co-culture with Lineage- Sca-1+ Kit+ (LSKs), biol rep2 |
| GSM1682820 |
UG26-1B6 stromal cells after one (d1; 24h) day of culture co-culture with Lineage- Sca-1+ Kit+ (LSKs), biol rep3 |
| GSM1682821 |
UG26-1B6 stromal cells after two (d2; 48h) day of culture co-culture with Lineage- Sca-1+ Kit+ (LSKs), biol rep1 |
| GSM1682822 |
UG26-1B6 stromal cells after two (d2; 48h) day of culture co-culture with Lineage- Sca-1+ Kit+ (LSKs), biol rep2 |
| GSM1682823 |
UG26-1B6 stromal cells after two (d2; 48h) day of culture co-culture with Lineage- Sca-1+ Kit+ (LSKs), biol rep3 |
| GSM1682824 |
UG26-1B6 stromal cells after three (d3; 72h) day of culture co-culture with Lineage- Sca-1+ Kit+ (LSKs), biol rep1 |
| GSM1682825 |
UG26-1B6 stromal cells after three (d3; 72h) day of culture co-culture with Lineage- Sca-1+ Kit+ (LSKs), biol rep2 |
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| Relations |
| BioProject |
PRJNA283870 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE68834_RAW.tar |
42.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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