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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 13, 2015 |
| Title |
Prmt5 is a crucial regulator of muscle stem cell expansion in adult mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Skeletal muscle stem cells (MuSC), also called satellite cells, are indispensable for maintenance and regeneration of adult skeletal muscles. Yet, a comprehensive picture of the regulatory events controlling the fate of MuSC is missing. Here, we determine the proteome of MuSC to design a loss-of-function screen, and identify 120 genes important for MuSC function including the arginine methyltransferase Prmt5. MuSC-specific inactivation of Prmt5 in adult mice prevents expansion of MuSC, abolishes long-term MuSC maintenance and abrogates skeletal muscle regeneration. Interestingly, Prmt5 is dispensable for proliferation and differentiation of Pax7(+) myogenic progenitor cells during mouse embryonic development, indicating significant differences between embryonic and adult myogenesis. Mechanistic studies reveal that Prmt5 controls proliferation of adult MuSC by direct epigenetic silencing of the cell cycle inhibitor p21. We reason that Prmt5 generates a poised state that keeps MuSC in a standby mode, thus allowing rapid MuSC amplification under disease conditions.
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| Overall design |
RNA from cultured satellite cells on Ion torrent sequencer
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| Contributor(s) |
Braun T, Kuenne C, Looso M, Kim J, Zhang T, Krüger M, Zhou Y, Günther S |
| Citation(s) |
26028225, 26484239 |
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| Submission date |
Mar 12, 2015 |
| Last update date |
Apr 01, 2020 |
| Contact name |
Mario Looso |
| E-mail(s) |
[email protected]
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| Organization name |
Max Planck Institute for Heart and Lung Research
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| Street address |
Ludwigstrasse 43
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| City |
Bad Nauheim |
| ZIP/Postal code |
61231 |
| Country |
Germany |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (4)
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| GSM1632699 |
cultured satellite cells PRMT5 KO Rep 2 |
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| Relations |
| BioProject |
PRJNA278041 |
| SRA |
SRP056106 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66822_PRMT5-vs-WT_SC-mrna_counts.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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