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| Status |
Public on Sep 17, 2015 |
| Title |
Frequent derepression of the mesenchymal transcription factor gene in acute myeloid leukemia (human) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Bone marrow samples from normal adult male donors were collected into EDTA. Red cells were removed by ammonium chloride lysis. Leukocytes were washed in SM buffer and CD34+ cells were separated from CD34- cells using an AutoMACS device and anti-CD34 immunomagnetic beads (Miltenyi Biotec), according to manufacturer’s instructions. For mature cell populations, CD34- cells were FACS purified according to the following immunophenotypes, with 7-AAD used to exclude dead cells: Neutrophils: side scatter high CD15+ CD16+. Monocytes: side scatter low-intermediate CD14+ CD16- CD15-. See also Huang et al., 2014.
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| |
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| Overall design |
Eight exon arrays total; 4 arrays for each cell type; four separate individuals.
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| |
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| Contributor(s) |
Somervaille T |
| Citation(s) |
26373280 |
| |
| Submission date |
Feb 24, 2015 |
| Last update date |
Sep 20, 2015 |
| Contact name |
Tim C Somervaille |
| E-mail(s) |
[email protected]
|
| Organization name |
Cancer Research UK Manchester Institute
|
| Lab |
Leukaemia Biology Laboratory
|
| Street address |
Wilmslow Road
|
| City |
Manchester |
| State/province |
Lancashire |
| ZIP/Postal code |
M20 4BX |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL5188 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE66256 |
Frequent derepression of the mesenchymal transcription factor gene in acute myeloid leukemia |
|
| Relations |
| BioProject |
PRJNA276269 |
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