|
| Status |
Public on Jan 24, 2015 |
| Title |
Transcriptome analysis of Wnt3a-treated triple-negative breast cancer cell lines. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We analyzed the transcriptome of two different triple negative breast cancer (TNBC) cell lines to define a comprehensive list of Wnt target genes. Cells were treated with Wnt3a for 6h, 12h or 24h. We found up-regulated and down-regulated genes in response to Wnt3a treatment. They are involved in the Wnt pathway itself, and also in TGFß, p53 and Hedgehog pathways. Thorough characterization of these novel potential Wnt target genes may reveal new regulators of the canonical Wnt pathway. The comparison of our list of Wnt target genes with those published in other cellular contexts confirms the notion that Wnt target genes are tissue-, cell line- and treatment-specific.
|
| |
|
| Overall design |
Cells were seeded in six-well plates, serum starved overnight then treated with Wnt3a for the indicated times (6, 12 and 24 hours). Triplicates for each condition were included in the experiment.
|
| |
|
| Contributor(s) |
Dubois T |
| Citation(s) |
25848952 |
| |
| Submission date |
Jan 23, 2015 |
| Last update date |
May 04, 2015 |
| Contact name |
Thierry Dubois |
| E-mail(s) |
[email protected]
|
| Organization name |
Institut Curie
|
| Department |
Recherche Center
|
| Lab |
Breast Cancer Biology Group
|
| Street address |
26 rue d’Ulm
|
| City |
PARIS |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL15640 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [CDF: Brainarray Entrez gene CDF v14.1, hugene11stv1hsentrezg] |
|
| Samples (36)
|
|
| Relations |
| BioProject |
PRJNA273488 |
Less...
More...