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| Status |
Public on Dec 20, 2007 |
| Title |
Analgesia of naloxone and morphine in wild-type and NY1DD sickle cell mice using BMAP cDNA microarrays |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Sickle cell disease is the most common genetic disorder in African-Americans. The opioid analgesic, morphine, has long been the treatment for the severe pain associated with this disease. Here we reveal that the opioid antagonist, naloxone, possesses potent analgesic activity in two strains of sickle cell mice (NY1DD and hBERK1) and not in their respective controls (ICR-CD1 and C57BL/6J) when administered by three parenteral routes. In the NY1DD sickle mice, naloxone (i.c.v.) possessed ~300-fold greater potency than morphine (i.c.v.). Other opioid antagonists (naltrexone, norbinaltorphimine, naltrindole) were substantially less effective in producing analgesia. Naloxone and morphine were synergistic in NY1DD mice, suggesting that analgesia was mediated via different receptor systems. Since microarray analysis suggested naloxone-induced down-regulation of the CCR5 chemokine receptor in NY1DD mice but not in control mice, the role of its endogenous ligand, CCL5 (RANTES), was investigated.
Keywords: Comparison of drug induced gene expression
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| Overall design |
In total 12 samples were analyzed. We used two strains of mice:
Wild-type: C57BL/6J
Sickle cell: NY1DD
To compare the gene expression patterns of naloxone and morphine, 4 treatments were used:
i) Naloxone on sickle cell mice - 3 replicates
ii) Morphiine on sickle cell mice - 3 replicates
iii) Naloxone on wild-type mice - 3 replicates
iv) Morphine on wild-type mice - 3 replicates
As we used dual channel cDNA microarrays, the experimental (Cy5) channel was used for the drugs (either morphine or naloxone) and the control (Cy3) was always saline treated.
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| Contributor(s) |
Yekkirala A, Lunzer MM, Hebbel RP, Portoghese PS |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Dec 07, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Ajay Yekkirala |
| Organization name |
University of Minnesota
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| Street address |
308 Harvard St 8-120
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| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55112 |
| Country |
USA |
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| Platforms (1) |
| GPL4464 |
UMN Mouse 11K V 11_22_04 BMAP |
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| Samples (12)
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| GSM141569 |
NY1DD Sickle Cell Mouse brains, NLX treated, 10mins. SCNLX-I 817 |
| GSM142384 |
NY1DD Sickle Cell Mouse brains, NLX treated, 10mins. 819 SCNLX2 |
| GSM142385 |
NY1DD Sickle Cell Mouse brains, NLX treated, 10mins. 825 SCNLX3 |
| GSM142386 |
NY1DD Sickle Cell Mouse brains, MOR treated, 10mins. 819 SCMOR1 |
| GSM142387 |
NY1DD Sickle Cell Mouse brains, MOR treated, 10mins. 823 SCMOR2 |
| GSM142388 |
NY1DD Sickle Cell Mouse brains, MOR treated, 10mins. 825 SCMOR3 |
| GSM148906 |
C57BL/6J wild-type mouse brains, MOR treated, 10mins. 916 WTMOR 1 |
| GSM148908 |
C57BL/6J wild-type mouse brains, MOR treated, 10mins. 916 WTMOR 2 |
| GSM148910 |
C57BL/6J wild-type mouse brains, MOR treated, 10mins. 925 WTMOR3 |
| GSM149076 |
C57BL/6J wild-type mouse brains, NLX treated, 10mins. 915 WTNLX 1 |
| GSM149078 |
C57BL/6J wild-type mouse brains, NLX treated, 10mins. 915 WTNLX 2 |
| GSM149084 |
C57BL/6J wild-type mouse brains, NLX treated, 10mins. 921 WTNLX 3 |
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| Relations |
| BioProject |
PRJNA98721 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6479_RAW.tar |
2.3 Mb |
(http)(custom) |
TAR (of GPS) |
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