 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 31, 2014 |
| Title |
SNP data from human fibroblasts and induced Pluripotent Stem cells from amyotrophic lateral sclerosis patients |
| Organism |
Homo sapiens |
| Experiment type |
SNP genotyping by SNP array
|
| Summary |
We have generated human induced Pluripotent Stem cells (hiPSc) from amyotrophic lateral sclerosis (ALS, motor neuron disease) patients, using Sendai virus-mediated delivery of reprogramming factors. hiPSc lines have been screened using SNP array to assess chromosomal stability (alongside the fibroblast lines from which they derived), and validation of the pluripotency of the hiPSc lines is provided by Pluritest assessment of transcriptome datasets, prior to differentiation to motor neuron cultures and downstream functional assays.
Mutihac R., Scaber J., Lalic T., Ababneh N., Vowles, J., Fletcher-Jones A., Douglas A.G.L., Browne C., Nakanishi M., Turner M., Wade-Martins R., Cowley S.A. and Talbot K. Altered ER calcium homeostasis and stress granule formation in iPSC-derived motor neurons from ALS/FTD patients with C9orf72 expansions. Submitted
|
| |
|
| Overall design |
human iPSc lines were derived from human dermal fibroblasts from 3 ALS patients with C9orf72 mutations (2 lines per patient) and control donors. SNP datasets for control fibroblasts HDF OX1 and NHDF, and SNP and gene expression datasets from control lines iPS OX1-19 and iPS NHDF-1, have been published previously: Van Wilgenburg B., Browne C., Vowles J. & Cowley S. A. Efficient, long term production of monocyte-derived macrophages from human pluripotent stem cells under partly-defined and fully-defined conditions. PLoS One 8, e71098 (2013). GEO Superseries GSE45472 ; Physiological characterisation of human iPS-derived dopaminergic neurons. Hartfield EM, Yamasaki-Mann M, Ribeiro Fernandes HJ, Vowles J, James WS, Cowley SA, Wade-Martins R.PLoS One. 2014 9(2):e87388 GEO superseries GSE43904. SNP dataset for control fibroblasts HDF AH017 is in press, and is available in GEO Superseries GSE69302.
|
| |
|
| Contributor(s) |
Mutihac R, Scaber J, Lalic T, Ababneh N, Vowles J, Fletcher-Jones A, Douglas AG, Browne C, Nakanishi M, Turner M, Wade-Martins R, Cowley SA, Talbot K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 30, 2014 |
| Last update date |
Dec 18, 2017 |
| Contact name |
Sally A Cowley |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0) 1865 275600
|
| Organization name |
University of Oxford
|
| Department |
Sir William Dunn School of Pathology
|
| Lab |
James Martin Stem Cell Facility
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3RE |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL13829 |
Illumina HumanCytoSNP-12 v2.1 BeadChip |
| GPL19699 |
HumanOmniExpress-24 v1.0 BeadChip |
|
| Samples (13)
|
|
| This SubSeries is part of SuperSeries: |
| GSE64584 |
Functional and pathological features of ALS in iPSC-derived motor neurons from patients with C9orf72 expansions |
|
| Relations |
| BioProject |
PRJNA271355 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE64582_RAW.tar |
72.2 Mb |
(http)(custom) |
TAR |
| GSE64582_signal_intensities_GPL13829.txt.gz |
32.1 Mb |
(ftp)(http) |
TXT |
| GSE64582_signal_intensities_GPL19699.txt.gz |
47.4 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...