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| Status |
Public on Feb 18, 2016 |
| Title |
Gene expression profiles of canonical and non-canonical NF-κB signaling pathways in Hodgkin’s lymphoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Malignant Hodgkin's lymphoma (HL) cells are characterized by constitutive activation of the canonical as well as the non-canonical NF-κB signaling cascades. We depleted subunit combinations corresponding to either canonical (p50/RelA) or non-canonical (p52/RelB) dimers in the HL cell line L-1236 and performed Affymetrix microarray analysis. Knockdown of p52/RelB affected the expression of a significantly higher number of genes than the knockdown of p50/RelA. The two sets of target genes presented a partial overlap, however they also revealed specific genes that are involved in distinct aspects of tumor biology.
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| Overall design |
The knockdown of subunit combinations corresponding to either canonical (p50/RelA, experimental group 1) or non-canonical (p52/RelB, experimental group 2) NF-κB heterodimers were carried out in L-1236 cells. Two distinct siRNA sequences for every NF-κB subunit and two non-targeting siRNA sequences (control) were used for each experimental group. Experiments were performed in biological triplicates.
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| Contributor(s) |
de Oliveira KA, Heinig M, Huebner N, Scheidereit C |
| Citation(s) |
26988706 |
| |
| Submission date |
Dec 16, 2014 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Claus Scheidereit |
| E-mail(s) |
[email protected]
|
| Phone |
+49 030 9406 3816
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| Organization name |
Max Delbrueck Center for Molecular Medicine
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| Department |
Signal Transduction in Tumor Cells
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| Street address |
Robert Roessle Str. 10
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| City |
Berlin |
| ZIP/Postal code |
13125 |
| Country |
Germany |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA270499 |
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