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| Status |
Public on Feb 13, 2017 |
| Title |
Roles of small RNAs in the effects of nutrition on apoptosis and spermatogenesis in the adult testis |
| Organism |
Ovis aries |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
In this study, we tested whether reduction in spermatozoal quality induced by under-nutrition is associated with an increase in germ cell apoptosis and a reduction in spermatogenesis, and whether these effects are regulated by small RNAs. Groups of 8 male sheep were fed for a 10% increase or 10% decrease in body mass over 65 days. Testicular tissue from underfed males had more apoptotic germ cells (TUNEL assay; P < 0.05) and greater (P < 0.05) levels of expression of apoptosis-related genes than well-fed males. We identified 44 miRNAs and 35 piRNAs that were differentially expressed in well-fed and underfed males (FDR < 0.05) and found that they were predominantly related to development of the reproductive system, apoptosis (miRNAs), or sperm production and quality (piRNAs). Furthermore, experimental validation showed that novel-miR-144, a homologue of miR-98, would target three apoptotic genes (TP53, CASP3, FASL). The proportion of miRNAs as a total of small RNAs was greater in well-fed males than in underfed males (P < 0.05) and was positively correlated with the proportion of piRNAs in well-fed males (r = 0.8, P < 0.05) and underfed males (r = 0.8, P < 0.05). We conclude that the reductions in spermatozoal quality induced by under-nutrition are due, at least partly, to increased germ cell apoptosis and to changes in the expression of miRNAs and piRNAs.
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| Overall design |
Two groups of 8 male sheep were fed for a 10% increase or 10% decrease in body mass over 65 days, and the small RNA expression was profiled.
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| Contributor(s) |
Guan Y, Liang G |
| Citation(s) |
25996545 |
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| Submission date |
Oct 29, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Guanxiang Liang |
| E-mail(s) |
[email protected]
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| Organization name |
University of Alberta
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| Street address |
410 Agriculture/Forestry Centre
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| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6G 2P5 |
| Country |
Canada |
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| Platforms (1) |
| GPL15670 |
Illumina HiSeq 2000 (Ovis aries) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA265167 |
| SRA |
SRP049367 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62797_RAW.tar |
400.0 Kb |
(http)(custom) |
TAR (of TXT) |
| GSE62797_miRNA-sequence.fa.gz |
3.8 Kb |
(ftp)(http) |
FA |
| GSE62797_piRNA-seqeunce.fa.gz |
168.0 Kb |
(ftp)(http) |
FA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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