 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 30, 2014 |
| Title |
Genome-wide omparism of Terc deficient ntESCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Haplo-insufficiency of telomerase genes in humans leads to telomere syndromes such as dyskeratosis congenital and idiopathic pulmonary fibrosis. Generation of pluripotent stem cells from telomerase haplo-insufficient donor cells would provide unique opportunities towards the realization of patient-specific stem cell therapies. Recently, pluripotent human embryonic stem cells (ntESCs) have been efficiently achieved by somatic cell nuclear transfer (SCNT). We tested the hypothesis that SCNT could effectively elongate shortening telomeres of telomerase haplo-insufficient cells in the ntESCs using relevant mouse models. Indeed, telomeres of telomerase haplo-insufficient (Terc+/-) mouse cells are elongated in ntESCs. Moreover, ntESCs derived from Terc+/- cells exhibit naïve pluripotency as evidenced by generation of Terc+/-ntESC clone pups by tetraploid embryo complementation (TEC), the most stringent test of naïve pluripotency. These data suggest that SCNT could offer a powerful tool to reprogram telomeres and to discover the factors for robust restoration of telomeres and pluripotency of telomerase haplo-insufficient somatic cells.
|
| |
|
| Overall design |
RNAs from Terc+/+, +/- and -/- contain 3 biological repeatsin each group.
|
| |
|
| Contributor(s) |
Sung L, Chang W, Zhang Q, Liu C, Liou J, Chang C, Ou-Yang H, Guo R, Fu H, Cheng WT, Ding S, Chen C, Okuka M, Keefe DL, Chen E, Liu L, Xu J |
| Citation(s) |
25464850 |
| |
| Submission date |
Oct 24, 2014 |
| Last update date |
Mar 04, 2015 |
| Contact name |
Li-Ying Sung |
| E-mail(s) |
[email protected]
|
| Phone |
+886-2-3366-6015
|
| Organization name |
National Taiwan University
|
| Department |
Institute of Biotechnology
|
| Lab |
Lab of Reproductive Biotechnology
|
| Street address |
4F, No. 81, Chang-Xing St.
|
| City |
Taipei |
| ZIP/Postal code |
106 |
| Country |
Taiwan |
| |
|
| Platforms (1) |
| GPL8389 |
Illumina Mouse-8 Expression BeadChip |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA264831 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62692_non-normalized.txt.gz |
866.1 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...