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| Status |
Public on Feb 20, 2015 |
| Title |
Splenic B cells from Hymenolepis diminuta-infected mice ameliorate colitis independent of T cells and via cooperation with macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Mouse infection with the tapeworm Hymenolepis diminuta leads to a less severe DNBS-colitis. Increased Th2 and regulatory cytokine production in the spleen is a hallmark of Hymenolepis diminuta infection, therefore we hypothesized that given this microenvironment, splenic adaptive cells acquire an anti-inflammatory phenotype. We tested the ability of putative splenic regulatory B cells generated by Hymenolepis diminuta infection to down-regulate intestinal inflammation. We found that unlike splenic B cells from uninfected mice, splenic B cells from Hymenolepis diminuta -infected animals ameliorated chemically-induced colitis.
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| Overall design |
Splenic B cells were magnetically isolated and also purified by cell sorting from 7 days-infected animals and B cells from uninfected animals were used as control.
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| Contributor(s) |
Reyes JL, Sigvardsson M, McKay DM |
| Citation(s) |
25452561 |
| |
| Submission date |
Sep 16, 2014 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Jose L Reyes |
| E-mail(s) |
[email protected]
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| Organization name |
The University of Calgary
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| Department |
Physiology and Pharmacology
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| Street address |
3330 Hospital Dr. NW
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| City |
Calgary |
| State/province |
Alberta |
| ZIP/Postal code |
T2N 4N1 |
| Country |
Canada |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA261161 |
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