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| Status |
Public on Jul 23, 2015 |
| Title |
Alpha-amylase overproduction in Streptomyces lividans |
| Platform organism |
Streptomyces coelicolor A3(2) |
| Sample organism |
Streptomyces lividans |
| Experiment type |
Expression profiling by array
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| Summary |
The gene aml encoding alpha-amylase in Streptomyces lividans was cloned in the multicopy plasmid pIJ486, generating plasmid pAMI11. Plasmid pAMI11 and pIJ486 were propagated in S. lividans TK21 to obtain S. lividans TK21(pAMI11) and its isogenic strain S. lividans TK21(pIJ486). Transcriptional profiling of the bacterium that overproduces alpha-amylase mainly resulted in the upregulation of genes involved in the biogenesis and function of ribosomes, together with the upregulation of the genes involved in the redox processes, the ABC transporters, the central carbon, aminoacid and purine /pyrimidine metabolism. Moreover, some genes involved in oxidative stress were upregulated. The number of genes downregulated was much lower than the upregulated ones. Therefore, bacteria respond by favouring alpha-amylase overproduction that apparently does not cause damage to the cell.
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| Overall design |
All microarray analyses were performed with RNA samples obtained from three independent cultures grown under identical conditions. The cDNA obtained from each RNA preparation of the alpha-amylase overproducer strain was hybridised with the cDNA obtained from the equivalent RNA preparation of the isogenic strain (S. lividans TK21 [pIJ486]).
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| Contributor(s) |
Gullón S, Mellado RP |
| Citation(s) |
26200356 |
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| Submission date |
Sep 16, 2014 |
| Last update date |
Jul 26, 2015 |
| Contact name |
Juan Carlos Oliveros |
| Organization name |
CNB, CSIC
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| Street address |
Darwin 3
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| City |
Cantoblanco |
| State/province |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
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| Platforms (1) |
| GPL8832 |
Eurogentec/CNB-CSIC Streptomyces coelicolor 17K TT-A220G |
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| Samples (3) |
| GSM1504998 |
Alpha-amylase overproducer versus wild type. REP1 |
| GSM1504999 |
Alpha-amylase overproducer versus wild type. REP2 |
| GSM1505000 |
Alpha-amylase overproducer versus wild type. REP3 |
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| Relations |
| BioProject |
PRJNA261120 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE61448_RAW.tar |
4.6 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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