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Series GSE59047 Query DataSets for GSE59047
Status Public on Apr 28, 2015
Title Regulation of tumor associated macrophages by cooperative inflammatory signaling
Organism Mus musculus
Experiment type Expression profiling by array
Summary Tumor associated macrophages show signs of both, classical pro-inflammatory as well as alternative macrophage activation. The aim of this study was to compare TAMs across tumor types, to characterize their phenotype in detail and to identify the signaling nodules involved regulating classical and alternative activation traits.
 
Overall design To identify gene expression profiles and pathways differentially activated in tumor-associated macrophages (TAMs) we isolated TAMs from in vivo animal models of EG7 thymoma (n=3), glioma (n=3) and neuroblastoma (n=3) by tumor digestion and subsequent enrichment of CD11b+ cells using Miltenyi magnetic beads. Thymomas grown in WT or Myd88-/- mice (n=5 per group) were used to identify TLR/IL1R dependent signaling in TAMs. RNA extracted from CD11b+ TAMs was analyzed using the Affymetrx HT_MG-430_PM arrays. For in-depth analysis of the role of inflammatory signaling by TNFR1 (Tnfrsf1a) and/or Myd88, EG7 TAM populations A (CD11b+, Ly6G-, Ly6C+, MHCII-) and C (CD11b+, Ly6C-, MHCII+) were isolated by flow cytometric sorting from WT, TNFR1-/-, Myd88flox/flox; Tie2-Cre and TNFR-/-; Myd88flox/flox; Tie2-cre mice and extracted RNA was analyzed by microarray using the Mouse Gene 2.0 GeneChip arrays (n=2 per genotype and population).
 
Contributor(s) Kratochvill F, Neale G, Murray P
Citation(s) 25500368
Submission date Jul 03, 2014
Last update date Aug 06, 2018
Contact name Geoffrey Neale
E-mail(s) [email protected]
Organization name St Jude Childrens Research Hospital
Department Hartwell Center
Street address 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
 
Platforms (2)
GPL11180 [HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (35)
GSM1424947 CD11b+ TAM isolated from neuroblastoma rep1
GSM1424948 CD11b+ TAM isolated from neuroblastoma rep2
GSM1424949 CD11b+ TAM isolated from neuroblastoma rep3
Relations
BioProject PRJNA254208

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Supplementary file Size Download File type/resource
GSE59047_RAW.tar 180.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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