|
| Status |
Public on Oct 17, 2014 |
| Title |
Genome-wide profiling of Zfp335 binding sites in thymocytes |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The generation of naïve T lymphocytes is critical for immune function yet the mechanisms governing their maturation remain incompletely understood. We have identified a mouse mutant, bloto, that harbors a hypomorphic mutation in the zinc finger protein Zfp335. Mutant blt/blt mice exhibit a naïve T cell deficiency due to an intrinsic developmental defect that begins to manifest in the thymus and continues into the periphery, affecting T cells that have recently undergone thymic egress. Zfp335 binds to promoter regions via a consensus motif, and its target genes are enriched in categories related to protein metabolism, mitochondrial function and transcriptional regulation. Restoring the expression of one target, Ankle2, partially rescues T cell maturation. Our findings identify Zfp335 as a transcription factor and essential regulator of late-stage intrathymic and post-thymic T cell maturation.
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| |
|
| Overall design |
4 Zfp335 ChIP-seq samples and 2 input samples with WT vs. blt/blt thymocytes.
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| |
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| Contributor(s) |
Han BY, Wu S, Foo CS, Horton RM, Jenne CN, Watson SR, Whittle B, Goodnow CC, Cyster JG |
| Citation(s) |
25343476, 27401554 |
| |
| Submission date |
Jun 09, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Brenda Yuyuan Han |
| Organization name |
Institute of Molecular and Cell Biology, A*STAR
|
| Street address |
61 Biopolis Drive, Proteos
|
| City |
Singapore |
| ZIP/Postal code |
138673 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE58293 |
Zinc finger protein Zfp335 is required for formation of the naïve T cell compartment |
|
| Relations |
| BioProject |
PRJNA252016 |
| SRA |
SRP043052 |
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