|
| Status |
Public on Jan 01, 2015 |
| Title |
MicroRNA expression profile in CHME3 cells infected with Japanese encephalitis virus |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
CHME3 cells grown on six-wells were infected with Japanese encephalitis virus (JEV) (P20778 strain), and total RNA was isolated from cells at 6, 24 and 48 h of post infection.
MicroRNA expression was significantly altered in JEV-infected human microglial cells. A time-dependent change in microRNA profile was noted. Bioinformatics analysis identified anti-correlation between differentially expressed miRNAs and the gene expression at different time point which ultimately affected several signalling pathways in microglia cells. Brain-enriched microRNAs and a set of microRNA previously designated as NeurimmiRs were also differentially expressed in response to JEV infection.
|
| |
|
| Overall design |
CHME3 cells grown on 6-well plate. Three replicates of uninfected and JEV-infected samples at each time point (6, 24 and 48 h of post infection) were used for microRNA array.
|
| |
|
| Contributor(s) |
Kumari B, Banerjee A, Vrati S, Trivedi AC |
| Citation(s) |
26838068 |
| |
| Submission date |
May 07, 2014 |
| Last update date |
Jul 03, 2019 |
| Contact name |
ARUP BANERJEE |
| E-mail(s) |
[email protected]
|
| Phone |
9650328080
|
| Organization name |
TRANSLATIONAL HEALTH SCIENCE & TECHNOLOGY INSTITUTE
|
| Department |
VIDRC
|
| Lab |
THSTI, VIDRC
|
| Street address |
THSTI, 3RD MILE STONE, FARIDABAD GURGAON EXPRESSWAY, NCR BIOTECH CLUSTER
|
| City |
FARIDABAD |
| State/province |
Haryana |
| ZIP/Postal code |
121001 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL16384 |
[miRNA-3] Affymetrix Multispecies miRNA-3 Array |
|
| Samples (18)
|
|
| This SubSeries is part of SuperSeries: |
| GSE57647 |
Time- and phase-dependent expression profile of miRNA and mRNA in human microglial cells (CHME3) infected with Japanese encephalitis virus (JEV) P20778 strain |
|
| Relations |
| BioProject |
PRJNA246404 |
Less...
More...