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| Status |
Public on Mar 21, 2022 |
| Title |
Esrrb Regulates Specific Feed-Forward Loops to Transit from Pluripotency into Early Stages of Differentiation |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Characterization of pluripotent states, in which cells can both self-renew or differentiate, with the irreversible loss of pluripotency, are important research areas in developmental biology. Although microRNAs (miRNAs) have been shown to be crucial for embryonic stem (ES) self-renewal maintenance and cellular differentiation, the role of miRNAs integrated into gene regulatory networks and its dynamic changes during these state transitions remain elusive. Here we describe the dynamic transcriptional regulatory circuitry of ES cells that incorporate protein-coding and miRNA genes based on microRNA array expression and quantitative sequencing of short transcripts upon the downregulation of the Estrogen Related Receptor Beta (Esrrb). The data reveals how Esrrb, a key stem cell transcription factor, regulates a specific ES cell miRNA expression program and integrates dynamic changes of feed forward loops contributing to the exit of the pluripotency state upon its downregulation. Together these findings provide new insights on the architecture of the combined transcriptional post-transcriptional regulatory network in stem cells.
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| Overall design |
Total of 12 murine embryonic stem cell line with controllable Esrrb expression (Esrrb_R) samples; Esrrb day0, Esrrb kd day1, Esrrb kd day3 and Esrrb kd Day5; all performed in triplicates
Please note that the common reference pool includes samples s1 to s3;
s1: Esrrb Day0 Rep1
s2: Esrrb Day0 Rep2
s3: Esrrb Day0 Rep3
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| Contributor(s) |
Mazloom A, Xu H, Reig JJ, Vasileva A, Ma’ayan A, Lemischka IR, Sevilla A |
| Citation(s) |
35652095 |
| |
| Submission date |
May 07, 2014 |
| Last update date |
Jun 20, 2022 |
| Contact name |
Ana Sevilla |
| E-mail(s) |
[email protected]
|
| Phone |
675348398
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| Organization name |
Universidad de Barcelona
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| Street address |
Avda Diagonal 643. Edificio Malagef 4rd Floor, Faculty of Biology
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| City |
Barcelona |
| State/province |
ESP |
| ZIP/Postal code |
08028 |
| Country |
Spain |
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| Platforms (1) |
| GPL18658 |
Exiqon miRCURY LNA microRNA Array 5th generation [condensed version; miRBASE v.14.0] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA246357 |
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