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| Status |
Public on May 01, 2014 |
| Title |
In Vitro Transformation of Primary Human CD34+ Cells by AML Fusion Oncogenes: Early Gene Expression Profiling Reveals Possible Drug Target in AML |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Different fusion oncogenes in acute myeloid leukemia (AML) have distinct clinical and laboratory features suggesting different modes of malignant transformation. Here we compare the in vitro effects of representatives of major groups of AML fusion oncogenes on primary human CD34+ cells.
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| Overall design |
For the 3 d and 8 d time points, the oncogenes were retrovirally expressed in primary human CD34+ cells, with cells transduced with empty retroviral vector as controls. As retroviral transduction requires days, for the 6 h time point the oncogenes were expressed from a pTracer-CMV/Bsd plasmid using nucleofection. Cells nucleofected with empty pTracer-CMV/Bsd vector were used as a control.
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| Contributor(s) |
Anmaar AM, Enas YR, Nabeel YR |
| Citation(s) |
20805992 |
| |
| Submission date |
Apr 30, 2014 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Anmaar Abdul-Nabi |
| E-mail(s) |
[email protected]
|
| Phone |
3127310297
|
| Organization name |
Washington University in Saint louis
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| Department |
Pathology and Immunology
|
| Lab |
Nabeel Yaseen
|
| Street address |
660 South Euclid Avenue. PO box 8118
|
| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA245884 |
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