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| Status |
Public on Oct 07, 2014 |
| Title |
Function of LIM-only protein FHL2 in vascular smooth muscle cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The LIM-only protein FHL2 is expressed in SMCs and inhibits SMC-rich lesion formation. However, the underlying mechanism behind FHL2's action in SMCs has been only partially resolved. To further elucidate the role of FHL2 in SMCs we compared the transcriptome of cultured SMCs derived from wild-type (WT) and FHL2-knockout (KO) mice.
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| Overall design |
Comparison of gene expression in aortic smooth muscle cells (SMCs) isolated from WT and FHL2-knockout (FHL2-KO) mice. SMCs isolated from three mouse aortas were pooled (Kurakula et al, PLOS One, 2014). Both for WT and FHL-KO mice three batches of SMCs (derived from 9 mice) were cultured. The SMCs were grown to confluency and maintained in serum-free medium for 48 hrs before harvest. Subsequently, RNA was isolated for gene expression profiling.
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| Contributor(s) |
Kurakula K, Moerland PD, van Tiel CM, de Vries CJ |
| Citation(s) |
25332231 |
| |
| Submission date |
Apr 16, 2014 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Perry D Moerland |
| E-mail(s) |
[email protected]
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| Organization name |
Amsterdam UMC
|
| Department |
Epidemiology and Data Science
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| Lab |
Bioinformatics Laboratory
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| Street address |
Meibergdreef 9
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| City |
Amsterdam |
| ZIP/Postal code |
1105AZ |
| Country |
Netherlands |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA244783 |
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