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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 22, 2015 |
| Title |
Jarid2 methylation is required for PRC2 functioning during ES cells differentiation |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report that Jarid2 is methylated at K116 by the polycomb complex PRC2. We analyzed Jarid2 versus Methylated Jarid2 genomic localization by ChIP-seq. We also study H3K27 enrichment in Jarid2 knock out ES cells and in cells rescued for Jarid2 or a mutant that cannot be methylated. ChIP-seq for H3K27me3 was perfomed in undiffrentiated ES cells as well as after 8 days of diffrentiation toward Embroyid Bodies (Hanging Drop method).
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| Overall design |
Experiment 1: Jarid2 vs Jarid2me ChIP-seq in undifferentiated mouse ES cells (E14). Experiment 2: H3K27me3 ChIP-seq in Jarid2 -/- ES cells (peng, 2009, Cell), or the same cells rescue with a BAC expressing JARID2 or a mutant of K116 to A. H3K27me3 Chip-seq were perfomed in undifferentiated ES cells and in EB after 8 days of differentiation (hanging Drop method).
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| Contributor(s) |
Teissandier A, Margueron R |
| Citation(s) |
25620564 |
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| Submission date |
Mar 05, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicolas Servant |
| E-mail(s) |
[email protected]
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| Organization name |
Institut Curie
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| Street address |
26 rue d'ulm
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| City |
Paris Cedex 05 |
| ZIP/Postal code |
75248 |
| Country |
France |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (16)
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| GSM1340606 |
Embryonic Stem cells Jarid2 -/- input |
| GSM1340607 |
Embryonic Stem cells Jarid2 -/- H3K27me3 IP, replicate 1 |
| GSM1340608 |
Embryonic Stem cells Jarid2 -/- H3K27me3 IP, replicate 2 |
| GSM1340609 |
Embryonic Stem cells Jarid2 -/- rescue with Jarid2 BAC H3K27me3 IP, replicate 1 |
| GSM1340610 |
Embryonic Stem cells Jarid2 -/- rescue with Jarid2 BAC H3K27me3 IP, replicate 2 |
| GSM1340611 |
Embryonic Stem cells Jarid2 -/- rescue with Jarid2K116A BAC H3K27me3 IP, replicate 1 |
| GSM1340612 |
Embryonic Stem cells Jarid2 -/- rescue with Jarid2K116A BAC H3K27me3 IP, replicate 2 |
| GSM1340613 |
Embryoid Bodies derived from mES cells Jarid2 -/- H3K27me3 IP, replicate 1 |
| GSM1340614 |
Embryoid Bodies derived from mES cells Jarid2 -/- H3K27me3 IP, replicate 2 |
| GSM1340615 |
Embryoid Bodies derived from mES cells Jarid2 -/- rescue with Jarid2 BAC H3K27me3 IP, replicate 1 |
| GSM1340616 |
Embryoid Bodies derived from mES cells Jarid2 -/- rescue with Jarid2 BAC H3K27me3 IP, replicate 2 |
| GSM1340617 |
Embryoid Bodies derived from mES cells Jarid2 -/- rescue with Jarid2 K116A BAC H3K27me3 IP, replicate 1 |
| GSM1340618 |
Embryoid Bodies derived from mES cells Jarid2 -/- rescue with Jarid2 K116A BAC H3K27me3 IP, replicate 2 |
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| Relations |
| BioProject |
PRJNA240247 |
| SRA |
SRP039457 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE55630_RAW.tar |
4.6 Gb |
(http)(custom) |
TAR (of BIGWIG, BROADPEAK, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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