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| Status |
Public on Mar 29, 2007 |
| Title |
Tissue-specific signature |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The experiments described here aim to generate a set of gene expression profiles from prostate, liver and kidney tissues using a custom-designed oligo-array platform enriched in messages that map to intronic regions of known human genes. Expression profiles of these tissues were combined in order to find a tissue-specific signature of intronic transcripts.
Keywords: Tissue-specific, Intronic transcripts
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| Overall design |
Equal amounts of 300ng of DNase-treated total RNA of each tissue sample (detailed below in section 2.3) were used to create amplified cyanine 3- and cyanine 5-labeled cRNA. Next, cRNA solutions were mixed and hybridized to oligo-array slide for 17 hours. Using this approach, we labeled 2 liver pool samples and 2 prostate samples, obtaining a total of 4 sets of microarray intensities from each tissue. Four sets of microarray intensities were also obtained by labeling 2 kidney pool samples with cy3 and 2 kidney pool samples with cy5.
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| Contributor(s) |
Nakaya HI, Amaral PP, Louro R, Reis EM, Almeida SV |
| Citation(s) |
17386095 |
| |
| Submission date |
Aug 03, 2006 |
| Last update date |
Oct 31, 2012 |
| Contact name |
Helder I. Nakaya |
| E-mail(s) |
[email protected]
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| Phone |
+551126481130
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| Organization name |
Universidade de São Paulo
|
| Lab |
csbiology.com
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| Street address |
AVENIDA PROFESSOR LINEU PRESTES, 580, Block 17
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| City |
SÃO PAULO |
| State/province |
SÃO PAULO |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
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| Platforms (1) |
| GPL4051 |
IQUSP_human_Intronic44k_v1.0 |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA95953 |
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