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| Status |
Public on Jan 30, 2014 |
| Title |
miR-638 promotes melanoma metastasis and protects melanoma cells from apoptosis and autophagy (microRNA) |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
MicroRNA (miRNA) expression profiling identified miR-638 as one of the most significantly overexpressed miRNAs in metastatic lesions compared with primary melanomas. miR-638 enhanced the tumourigenic properties of melanoma cells in vitro and lung colonization in vivo. mRNA expression profiling of miR-638 and antagomir-transduced cells identified new candidate genes as miR-638 targets, the majority of which is involved in p53-mediated apoptosis regulation. miR-638 depletion stimulated expression of p53 and its downstream target genes and induced apoptosis and autophagy in melanoma cells. miR-638 promoter analysis revealed transcription factor associated protein 2-α (TFAP2A) as a direct negative regulator of miR-638. Further analyses provided strong evidence for a double negative regulatory feedback loop between miR-638 and TFAP2A. Taken together, miR-638 may support melanoma progression by suppressing p53-mediated apoptosis pathways and by targeting the transcriptional repressor TFAP2A.
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| Overall design |
TaqMan® low-density arrays (TLDA; human microRNA Cards A v2.1 & B v2.0, Applied Biosystems, Darmstadt, Germany) were used for measuring the expression of 667 human miRNAs in primary melanomas (PM, n=8), lymph node metastases (LNM, n=9) or distant cutaneous metastases (DCM, n=10).
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| Contributor(s) |
Bhattacharya A, Schmitz U, Raatz Y, Schönherr M, Kottek T, Schauer M, Franz S, Saalbach A, Anderegg U, Wolkenhauer O, Schadendorf D, Simon JC, Magin T, Vera J, Kunz M |
| Citation(s) |
25650662 |
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| Submission date |
Jan 29, 2014 |
| Last update date |
May 26, 2016 |
| Contact name |
Animesh Bhattacharya |
| E-mail(s) |
[email protected]
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| Organization name |
University of Leipzig, Medical Faculty
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| Department |
Dermatology
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| Lab |
AG. Kunz
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| Street address |
Johannisallee 30, MBFZ, Room no B-214
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| City |
Leipzig |
| State/province |
Saxony |
| ZIP/Postal code |
04103 |
| Country |
Germany |
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| Platforms (1) |
| GPL10262 |
Renji Hospital ABI Human TaqMan® MicroRNA Assays V2.0 |
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| Samples (25)
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| GSM1316763 |
Cryopreserved patient material-primary_malignant_melanoma_sample1 |
| GSM1316764 |
Cryopreserved patient material-primary_malignant_melanoma_sample4 |
| GSM1316765 |
Cryopreserved patient material-primary_malignant_melanoma_sample2 |
| GSM1316766 |
Cryopreserved patient material-primary_malignant_melanoma_sample5 |
| GSM1316767 |
Cryopreserved patient material-primary_malignant_melanoma_sample8 |
| GSM1316768 |
Cryopreserved patient material-primary_malignant_melanoma_sample7 |
| GSM1316769 |
Cryopreserved patient material-primary_malignant_melanoma_sample6 |
| GSM1316770 |
Cryopreserved patient material-primary_malignant_melanoma_sample3 |
| GSM1316771 |
Cryopreserved patient material-lymph_node_metastasis_sample3 |
| GSM1316772 |
Cryopreserved patient material-lymph_node_metastasis_sample6 |
| GSM1316773 |
Cryopreserved patient material-lymph_node_metastasis_sample4 |
| GSM1316774 |
Cryopreserved patient material-lymph_node_metastasis_sample2 |
| GSM1316775 |
Cryopreserved patient material-lymph_node_metastasis_sample5 |
| GSM1316776 |
Cryopreserved patient material-lymph_node_metastasis_sample9 |
| GSM1316777 |
Cryopreserved patient material-lymph_node_metastasis_sample7 |
| GSM1316778 |
Cryopreserved patient material-lymph_node_metastasis_sample8 |
| GSM1316779 |
Cryopreserved patient material-lymph_node_metastasis_sample1 |
| GSM1316780 |
Cryopreserved patient material-distant_cutaneous_metastasis_sample9 |
| GSM1316781 |
Cryopreserved patient material-distant_cutaneous_metastasis_sample1 |
| GSM1316782 |
Cryopreserved patient material-distant_cutaneous_metastasis_sample6 |
| GSM1316783 |
Cryopreserved patient material-distant_cutaneous_metastasis_sample4 |
| GSM1316784 |
Cryopreserved patient material-distant_cutaneous_metastasis_sample5 |
| GSM1316785 |
Cryopreserved patient material-distant_cutaneous_metastasis_sample10 |
| GSM1316786 |
Cryopreserved patient material-distant_cutaneous_metastasis_sample3 |
| GSM1316787 |
Cryopreserved patient material-distant_cutaneous_metastasis_sample2 |
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| This SubSeries is part of SuperSeries: |
| GSE54494 |
miR-638 promotes melanoma metastasis and protects melanoma cells from apoptosis and autophagy |
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| Relations |
| BioProject |
PRJNA236647 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE54492_fold_change.txt.gz |
13.6 Kb |
(ftp)(http) |
TXT |
| GSE54492_non-normalized.txt.gz |
60.3 Kb |
(ftp)(http) |
TXT |
| GSE54492_normalized.txt.gz |
69.4 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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