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| Status |
Public on Dec 20, 2008 |
| Title |
The transcriptional changes induced by AtLEJ1 gene expression |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The loss-of-function mutant of AtLEJ1 had well-developed anther cells and showed an increased accumulation of JA and ET in its flower bud clusters. Conversely, overexpression of AtLEJ1 caused a delay in the development of the floral organs, arrested anther cell development and secondary cell-wall biosynthesis in the endothecium cells, and reduced JA and ET contents in the flower bud clusters, resulting in male-sterility through anther indehiscence.
We used microarrays to gain a comprehensive understanding of the transcriptional changes induced by AtLEJ1 gene expression,
Keywords: Comparison of transcripts during flower development among 35S:AtLEJ1, atlej1 and wild type.
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| Overall design |
We performed a full genome microarray analysis (ATH1 Affymetrix chips) with the wild type, 35S:AtLEJ1/5-8 line and atlej1 mutant. Total RNAs were obtained separately from unopened flower buds at stages 1~12, when the anthers are not yet opened, and from open flowers at stages 13~14, when anthesis occurs.
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| Contributor(s) |
Shin JS, Jung KW, Yoo KS, Kim YY, Cui MH |
| Citation missing |
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| Submission date |
Aug 03, 2006 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Jung Kwang Wook |
| E-mail(s) |
[email protected]
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| Organization name |
Korea university
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| Street address |
Anam-dong 1-ga
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| City |
Seoul |
| ZIP/Postal code |
136-701 |
| Country |
South Korea |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (18)
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| GSM124896 |
OE-OF, biological rep1 |
| GSM124897 |
KO-B, biological rep1 |
| GSM124898 |
KO-OF, biological rep1 |
| GSM153864 |
WT-B, biological rep2 |
| GSM153865 |
WT-B, biological rep3 |
| GSM153866 |
WT-OF, biological rep2 |
| GSM153867 |
WT-OF, biological rep3 |
| GSM153868 |
OE-B, biological rep2 |
| GSM153869 |
OE-B, biological rep3 |
| GSM153870 |
OE-OF, biological rep2 |
| GSM153871 |
OE-OF, biological rep3 |
| GSM153872 |
KO-B, biological rep2 |
| GSM153873 |
KO-B, biological rep3 |
| GSM153874 |
KO-OF, biological rep2 |
| GSM153875 |
KO-OF, biological rep3 |
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| Relations |
| BioProject |
PRJNA95945 |
| Supplementary data files not provided |
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