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| Status |
Public on Jun 17, 2014 |
| Title |
Expression data from rGRF3, 35S:GIF1 and rGRF3x35S:GIF1 plants |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
The Growth Regulating Factors (GRFs) are plant specific transcription factors. They form complexes with GRF Interacting Factors (GIFs), a small family of transcriptional co-activators. In Arabidopsis thaliana, seven out of the nine GRFs are regulated by microRNA miR396. A detailed analysis of GRF3 revealed that a modified transgene, insensitive to the regulation of miR396, causes a strong increase in the number of cells in leaves, while an additional increase of GIF1 expression further enhances the number of cells synergistically. Genome-wide transcript profiling revealed that simultaneous increase of GRF3 and GIF1 levels causes additional effects in gene expression compared to either of the transgenes alone. We observed that GIF1 interacts in vivo with GRF3, as well as chromatin remodeling complexes, providing a mechanistic explanation for the additional activities of a GRF3-GIF1 complex. Interestingly, we found that the GRF system also regulates leaf longevity. Genetic and molecular analysis revealed that the functions of GRFs in leaf size and senescence can be uncoupled, demonstrating that the GRFs control different stages of leaf development. The results provide new insights into the functions of a complex regulatory network composed of microRNAs, transcription factors, and co-transcription factors.
We used microarrays to obtain the transcription profile of plants overexpressing GRF3, GIF1 or both genes toghether.
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| Overall design |
We compared the transcriptome of 35S:GIF1, rGRF3 and rGRF3x35S:GIF1 plants vs wild-type plants (Col-0 accession), using ATH1 Affymetrics microarrays. For these experiments we collected vegetative apices together with the leaf primordia from plants of 20 days growth in short-day. For each genotype (Col-0, rGRF3, 35S:GIF1 and rGRF3x35S:GIF1) we collected two samples.
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| Contributor(s) |
Debernadi JM, Palatnik JF |
| Citation(s) |
24888433 |
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| Submission date |
Dec 18, 2013 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Juan Manuel Debernardi |
| E-mail(s) |
[email protected]
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| Organization name |
IBR
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| Street address |
Ocampo y Esmeralda
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| City |
Rosario |
| State/province |
Santa Fe |
| ZIP/Postal code |
2000 |
| Country |
Argentina |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA231991 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53438_RAW.tar |
15.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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