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| Status |
Public on Jun 01, 2014 |
| Title |
MicroRNAs in an Experimental Model of Physiologic Left Ventricular Hypertrophy: a Microarray-based Analysis |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
BACKGROUND - MicroRNAs (miRs) are a class of small non-coding RNAs that regulate gene expression. Transgenic models have proved that a single miR can induce pathological cardiac hypertrophy and failure. The roles of miRs in the genesis of physiologic left ventricular hypertrophy (LVH), however, are not well elucidated. OBJECTIVE - To evaluate miRs expression in an experimental model of exercise-induced LVH. METHODS - Male Balb/c mice were divided into sedentary (SED) and exercise (EXE) groups. Voluntary exercise was performed in odometer-monitored metal wheels during 35 days. Analyses were performed after 7 and 35 days of training, and consisted of transthoracic echocardiography, maximal exercise test, miRs microarray (miRBase v.16) and real-time qRT-PCR analysis. RESULTS - Left ventricular weight/body weight ratio increased by 7% in the EXE group at day 7 (p<0.01) and by 11% at 35 days of training (p<0.001) After 7 days of training, microarray identified 35 deregulated miRs: 20 had an increase in their expression and 15 were down-regulated (p=0.01). At day 35 of training, 25 miRs were deregulated: 15 were up-regulated and 10 had decreased their expression compared to the SED group (p<0.01). qRT-PCR confirmed an increase in miR-150 levels at both time points and a decrease in miR-26b, miR-27a and miR-143 after 7 days of voluntary exercise. CONCLUSIONS – We unraveled new miRs that can modulate physiological cardiac hypertrophy, particularly miR-26b, -150, 27a and -143. Our data also indicate that previously established regulatory gene pathways involved in pathological LVH are not deregulated in physiologic LVH.
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| Overall design |
Experimental model of left ventricular hypertrophy induced by voluntary exercise
Male Balb/c mice, 8-10 weeks old, 4 groups analyzed, each group consisted of a pool from 4 animals
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| Contributor(s) |
Martinelli NC, Cohen CR, dos Santos KG, Biolo A, Frick LM, Silvello DN, Schneider S, Andrades ME, Clausell N, Matte U, Rohde LE |
| Citation(s) |
24751578 |
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| Submission date |
Nov 12, 2013 |
| Last update date |
Sep 11, 2014 |
| Contact name |
Nidiane Carla Martinelli |
| E-mail(s) |
[email protected]
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| Organization name |
Federal University of Rio Grande do Sul
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| Department |
Cardiology Division at Hospital de Clínicas de Porto Alegre
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| Lab |
Cardiovascular Experimental and Molecular Laboratory
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| Street address |
AV ALEGRETE, 414/202
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| City |
Porto Alegre |
| State/province |
Rio Grande do Sul |
| ZIP/Postal code |
90460-100 |
| Country |
Brazil |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA227330 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE52278_RAW.tar |
280.0 Kb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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