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| Status |
Public on Apr 25, 2014 |
| Title |
WRKY6 Transcription Factor Restricts Arsenate Uptake and Transposon Activation in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Stress constantly challenges plant adaptation to the environment. Of all stress types, arsenic was a major threat during the early evolution of plants. The most prevalent chemical form of arsenic is arsenate, whose similarity to phosphate renders it easily incorporated into cells via the phosphate transporters. Here we found that arsenate stress provokes a notable transposon burst in plants, in coordination with arsenate/phosphate transporter repression, which immediately restricts arsenate uptake. This repression was accompanied by delocalization of the phosphate transporter from the plasma membrane. When arsenate was removed, the system rapidly restored transcriptional expression and membrane localization of the transporter. We identify WRKY6 as an arsenate-responsive transcription factor that mediates arsenate/phosphate transporter gene expression and restricts arsenate-induced transposon activation. Plants therefore have a dual WRKY-dependent signaling mechanism that modulates arsenate uptake and transposon expression, providing a coordinated strategy for arsenate tolerance and transposon gene silencing.
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| Overall design |
Three biological replicates were performed for each sample type. Single channel hybridizations were carried-out using either Affymetrix ATH1 platform or Nimblegen Gene Expression 12x135K platform (Arabidopsis thaliana).
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| Contributor(s) |
Castrillo G, Sánchez-Bermejo E, de Lorenzo L, Crevillen P, Fraile-Escanciano A, TC M, Mouriz A, Catarecha P, Sobrino-Plata J, Olsson S, Leo del Puerto Y, Mateos I, Rojo E, Hernández LE, Jarillo JA, Piñeiro M, Paz-Ares J, Leyva A |
| Citation(s) |
23922208 |
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| Submission date |
Jul 19, 2013 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Juan Carlos Oliveros |
| Organization name |
CNB, CSIC
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| Street address |
Darwin 3
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| City |
Cantoblanco |
| State/province |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
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| Platforms (2) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
| GPL13970 |
NimbleGen Arabidopsis thaliana Gene Expression 12x135K Array [100718_Athal_TAIR9_exp; SEQ_ID] |
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| Samples (21)
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| GSM1192719 |
Wild type, control (not supplemented). Rep1 |
| GSM1192720 |
Wild type, control (not supplemented). Rep2 |
| GSM1192721 |
Wild type, control (not supplemented). Rep3 |
| GSM1192722 |
Wild type, 30μM arsenate, 8 hours. Rep1 |
| GSM1192723 |
Wild type, 30μM arsenate, 8 hours. Rep2 |
| GSM1192724 |
Wild type, 30μM arsenate, 8 hours. Rep3 |
| GSM1192725 |
Wild type, 30μM phosphate, 8 hours. Rep1 |
| GSM1192726 |
Wild type, 30μM phosphate, 8 hours. Rep2 |
| GSM1192727 |
Wild type, 30μM phosphate, 8 hours. Rep3 |
| GSM1192728 |
Wild type, 1mM phosphate, 8 hours. Rep1 |
| GSM1192729 |
Wild type, 1mM phosphate, 8 hours. Rep2 |
| GSM1192730 |
Wild type, 1mM phosphate, 8 hours. Rep3 |
| GSM1192731 |
Wild type, control (not supplemented). Rep1 |
| GSM1192732 |
Wild type, control (not supplemented). Rep2 |
| GSM1192733 |
Wild type, control (not supplemented). Rep3 |
| GSM1192734 |
Wild type, 30μM arsenate, 1.5 hours. Rep1 |
| GSM1192735 |
Wild type, 30μM arsenate, 1.5 hours. Rep2 |
| GSM1192736 |
Wild type, 30μM arsenate, 1.5 hours. Rep3 |
| GSM1192737 |
Plants overexpressing WRKY6, arsenate 30μM, 1.5 hours. Rep1 |
| GSM1192738 |
Plants overexpressing WRKY6, arsenate 30μM, 1.5 hours. Rep2 |
| GSM1192739 |
Plants overexpressing WRKY6, arsenate 30μM, 1.5 hours. Rep3 |
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| Relations |
| BioProject |
PRJNA212734 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE49037_RAW.tar |
54.9 Mb |
(http)(custom) |
TAR (of CEL, PAIR) |
| Processed data included within Sample table |
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