|
| Status |
Public on Jul 28, 2014 |
| Title |
TRIM28 regulates RNA polymerase II promoter proximal pausing and entry into processive elongation |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Analysis of total PolII and Ser2-phosphorylated PolII genomic occupancy in control-transfected (WT) or Trim28-knockdown (Trim28-KD) embryonic stem cells.
|
| |
|
| Overall design |
Embryonic stem cells were transfected with luciferase siRNA (WT) or Trim28 siRNA (Trim28 KD) in duplicate experiments. 48 hrs after transfection, cells were crosslinked with formaldehyde, collected, lysed, and chromatin was fragmented with sonication. Total PolII or Ser2-phosphorylated PolII genomic occupancy in WT or Trim28 KD cells were determined by ChIP-seq.
|
| |
|
| Contributor(s) |
Bunch H, Zheng X, Burkholder A, Motola S, Dillon ST, Ebmeier CC, Levine S, Fargo D, Hu G, Taatjes DJ, Calderwood SK |
| Citation(s) |
25173174, 27432546 |
| |
| Submission date |
Jun 24, 2013 |
| Last update date |
May 29, 2019 |
| Contact name |
David Fargo |
| E-mail(s) |
[email protected]
|
| Organization name |
National Institute of Environmental Health Sciences
|
| Department |
HHS
|
| Lab |
Integrative Bioinformatics
|
| Street address |
P.O.Box 12233
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA209380 |
| SRA |
SRP026280 |
Less...
More...