 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 23, 2015 |
| Title |
Whole blood gene expression profiles distinguish clinical phenotypes of venous thromboembolism [Set1] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Recurrent venous thromboembolism (VTE) occurs infrequently following a provoked event but occurs in up to 30% of individuals following an initial unprovoked event. We studied 134 patients with VTE separated into 3 groups: (1) ‘low-risk’ patients had ≥1 provoked VTE; (2) ‘moderate-risk’ patients had no more than 1 unprovoked VTE; (3) ‘high-risk’ patients had ≥2 unprovoked VTE. 44 individuals with no history of VTE were enrolled as healthy controls. Consented individuals were enrolled at 4 medical centers in the US. Total RNA from whole blood was isolated and hybridized to Illumina HT-12 V4 Beadchips to assay whole genome expression. Using class prediction analysis, we distinguished high-risk patients from healthy controls with good receiver operating curve characteristics (AUC=0.88). We also distinguished high-risk from low-risk individuals, moderate-risk individuals from healthy controls, and low-risk individuals from healthy controls with AUC’s of 0.72, 0.77 and 0.72, respectively. Using differential expression analysis, we identified genes relevant to coagulation, immune response and vascular biology, such as SELP and CD46, which were differentially expressed in at least two comparisons. Neither approach distinguished the moderate-risk patients from the high-risk or low-risk groups. Gene expression profiles may provide insights into biological mechanisms associated with patients at risk for recurrent VTE. Prospective studies are needed to validate these findings.
|
| |
|
| Overall design |
This study includes a total of 218 samples/individuals (in 5 groups; APS, high-risk VTE, moderate-risk VTE, low-risk VTE and healthy-controls). Samples in which the percent of probes present was 15% or less (n=51) were excluded leaving 167 samples. The data for these 167 samples were normalized together. However, this record represents the 132 individual samples in the following groups; high-risk (n=40); moderate-risk (n=33); low-risk (n=34); and healthy controls (n=25). The 35 samples in APS group are represented in GSE48001.
|
| |
|
| Contributor(s) |
Lewis DA, Suchindran S, Beckman MG, Hooper WC, Grant AM, Heit JA, Manco-Johnson M, Moll S, Philipp CS, Kenney K, deStaercke C, Pyle ME, Lucas JE, Chi J, Ortel TL |
| Citation(s) |
25684211 |
| |
| Submission date |
Jun 17, 2013 |
| Last update date |
Apr 12, 2022 |
| Contact name |
Deborah A Lewis |
| E-mail(s) |
[email protected]
|
| Organization name |
Duke University Medical Center
|
| Street address |
201 Trent Dr
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (132)
|
|
| Relations |
| BioProject |
PRJNA208790 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48000_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE48000_non_normalized_167samples.txt.gz |
17.5 Mb |
(ftp)(http) |
TXT |
| GSE48000_non_normalized_set1.txt.gz |
13.9 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...