|
| Status |
Public on Jan 01, 2014 |
| Title |
Diversity of HrpL-regulons across Pseudomonas syringae isolates |
| Organism |
Pseudomonas syringae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We implemented transcriptional analysis methods using cDNA and high-throughput sequencing data to identify HrpL-regulated genes for six strains of Pseudomonas syringae
|
| |
|
| Overall design |
Each Pseudomonas syringae strains was transformed with either pBAD::EV or pBAD containing native hrpL sequence. Strains were grown in MM media supplemented with arabinose and collected 1, 3, and 5 hours post arabinose treatment. RNA was extracted for each time point and mixed at a 1/3 ratio. After removal of rRNA, double stranded cDNA was generated and library prepared accordeing to Illumina protocols.
|
| |
|
| Contributor(s) |
Mucyn TS, Lind AL, Biswas S, Yoursatone S, Nishimura MT, Cumbie JS, Grant SR, Chang JH, Jones CD, Dangl JL |
| Citation(s) |
24391493 |
| |
| Submission date |
May 14, 2013 |
| Last update date |
Aug 08, 2019 |
| Contact name |
Emma Johnson |
| E-mail(s) |
[email protected]
|
| Organization name |
University of North Carolina - Chapel Hill
|
| Department |
Biology
|
| Lab |
Dangl Lab
|
| Street address |
250 Bell Tower Dr
|
| City |
Chapel Hill |
| State/province |
North Carolina |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17159 |
Illumina Genome Analyzer II (Pseudomonas syringae) |
| GPL17160 |
Illumina HiSeq 2000 (Pseudomonas syringae) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA203106 |
| SRA |
SRP022745 |
Less...
More...