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| Status |
Public on Jan 20, 2015 |
| Title |
Candidatus pelagibacter ubique HTCC1062 requires 4-amino-5-hydroxymethyl-2-methylpyrimidine, an abundant thiamine precursor in the sea |
| Platform organisms |
Candidatus Pelagibacter ubique HTCC1002; Candidatus Pelagibacter ubique HTCC1062; Candidatus Pelagibacter sp. HTCC7211 |
| Sample organism |
Candidatus Pelagibacter ubique HTCC1062 |
| Experiment type |
Expression profiling by array
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| Summary |
Thiamine is often undetectable in ocean surface waters where Pelagibacter cells are numerically abundant. Despite this, Pelagibacter cells are missing de novo thiamine synthesis pathways. We show that an eogenous source of the thiamine precursor HMP is required for thiamine synthesis in Pelagibacter and that this precursor is abundant in the Sargasso sea.
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| Overall design |
Batch cultures of P. ubique were grown in a defined arificial seawater media. Three cultures were given no thiamine amendment, and three other cultures received an excess concentration of thiamine. Cultures were harvested for microarray analyses just prior to and after thiamine limitation for the purpose of observing differences in gene expression related to thiamine limitation.
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| Contributor(s) |
Carini P, Giovannoni SJ |
| Citation missing |
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| Submission date |
May 06, 2013 |
| Last update date |
Jan 20, 2015 |
| Contact name |
Paul Carini |
| Organization name |
Oregon State U
|
| Street address |
220 NASH hall
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| City |
Corvallis |
| ZIP/Postal code |
97331 |
| Country |
USA |
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| Platforms (1) |
| GPL7330 |
Candidatus Pelagibacter ubique OSU_SAR11_affychip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA201488 |
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