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| Status |
Public on Sep 30, 2013 |
| Title |
Genome-wide analysis of BRCA1 and PALB2 [TNF-alpha stimulation] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Breast and ovarian cancer susceptibility genes, BRCA1 and PALB2 have enigmatic roles in cellular growth and mammalian development. While these genes are essential for growth during early developmental programs, inactivation later in adulthood leads to increased growth and formation of tumors, leading to their designation as tumor suppressors. We performed genome-wide analysis assessing their chromatin residence and gene expression responsiveness using high throughput sequencing in breast epithelial cells. These experiments revealed a critical role for BRCA1 and PALB2 in transcriptional responsiveness to NF-kB, a crucial mediator of growth and inflammatory response during development and cancer. Importantly, we also uncovered a vital role for these proteins in response to retinoic acid (RA), a growth inhibitory signal in breast cancer cells, which may constitute the basis for their tumor suppressor activity.
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| Overall design |
MCF-7 cells were infected with PALB2, BRCA1, p65/RelA and non targeting (SCR) shRNAs. After puromycin selection, cells were stimulated with 10ng/ml TNF-alpha 1 hour
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| Contributor(s) |
Gardini A, Cesaroni M, Shiekhattar R |
| Citation(s) |
24591564 |
| |
| Submission date |
Apr 02, 2013 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Alessandro Gardini |
| E-mail(s) |
[email protected]
|
| Phone |
305-243-7394
|
| Organization name |
University of Miami
|
| Department |
Sylvester Comprehensive Cancer Center
|
| Lab |
Ramin Shiekhattar
|
| Street address |
1501 NW 10 Avenue
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE45715 |
Genome-wide analysis of BRCA1 and PALB2 |
|
| Relations |
| BioProject |
PRJNA195927 |
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