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| Status |
Public on Feb 21, 2013 |
| Title |
Genomic changes in low vs high metastatic A549 NSCLC cell line |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by SNP array
SNP genotyping by SNP array
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| Summary |
Genomic changes in low and highly metastatic A549 cells were analyzed by 500K SNP arrays. A large number of genomic alterations were present in A549 cells but no significant differences were observed between the low or highly metastatic A549 cell lines.
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| Overall design |
We generated a NSCLC line with highly increased propensity to form tumor nodules in murine lungs after intravenous injections. Extravasation and growth at a distant site are important parts of the metastatic process and we regarded these as a surrogate marker for in vivo aggressiveness and potential metastatic capability. A549 lung asdenocarcimona cell line with initially low metastatic potential was used for this purpose; these cells formed multiple small nodules in NOD/SCID mice after first i.v.-injection, round 1 (R1). Removal of tumor nodules from the lungs and subsequent re-injection led to a rapid increase in metastatic capacity. A highly aggressive phenotype which was stable over time was evident after three rounds (R3) of in vivo selection for the A549 cell line.
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| Contributor(s) |
Thoennissen NH |
| Citation missing |
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| Submission date |
Feb 21, 2013 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Nils H Thoennissen |
| E-mail(s) |
[email protected]
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| Organization name |
University Hospital of Münster
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| Department |
Hematology Oncology
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| Street address |
Albert-Schweitzer-Str. 33
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| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
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| Platforms (1) |
| GPL6801 |
[GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array |
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| Samples (2) |
| GSM1086289 |
A549 cell line, round 0, before in vivo injection |
| GSM1086290 |
A549 cell line, round 3, after 3 times in vivo injection |
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| Relations |
| BioProject |
PRJNA190053 |
