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| Status |
Public on Feb 22, 2013 |
| Title |
H3K9me2-Ab2 (new lot).D.mel 3rd Instar Larvae Nuclei EGG Mutant |
| Project |
modENCODE
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| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
modENCODE_submission_3692
This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
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| Overall design |
EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: EGG mutant
Developmental Stage: 3rd Instar Larvae; Genotype: EGG [10.1-1a]/EGG [10.1-1a]; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain EGG mutant
Antibody H3K9me2-Ab2 (new lot) (target is H3K9me2); Developmental Stage 3rd Instar Larvae
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| Web link |
http://www.modencode.org
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| Contributor(s) |
Karpen G, Elgin S, Gortchakov A, Shanower G, Tolstorukov M, Kharchenko P, Kuroda M, Pirrotta V, Park P, Minoda A, Riddle N, Schwartz Y, Alekseyenko A, Kennedy C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| BioProject |
PRJNA63463 |
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| Submission date |
Feb 21, 2013 |
| Last update date |
Jul 08, 2015 |
| Contact name |
DCC modENCODE |
| E-mail(s) |
[email protected]
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| Phone |
416-673-8579
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| Organization name |
Ontario Institute for Cancer Research
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| Lab |
modENCODE DCC
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| Street address |
MaRS Centre, South Tower, 101 College Street, Suite 800
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| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
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| Platforms (1) |
| GPL6629 |
[DM_tiling2_MR] Affymetrix Drosophila Tiling 2.0R Array |
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| Samples (4)
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| GSM1085783 |
H3K9me2-Ab2 (new lot) Nuclear L3 EGG D.melanogaster Input Rep1 |
| GSM1085784 |
H3K9me2-Ab2 (new lot) Nuclear L3 EGG D.melanogaster Input Rep2 |
| GSM1085785 |
H3K9me2-Ab2 (new lot) Nuclear L3 EGG D.melanogaster ChIP Rep1 |
| GSM1085786 |
H3K9me2-Ab2 (new lot) Nuclear L3 EGG D.melanogaster ChIP Rep2 |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE44469_RAW.tar |
173.8 Mb |
(http)(custom) |
TAR (of BEDGRAPH, CEL) |
| GSE44469_repset.14517403.smoothedM.bedgraph.gz |
41.5 Mb |
(ftp)(http) |
BEDGRAPH |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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